MA104

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85102918, African green monkey kidney, Epithelial

• eCl@ss: 32190102
• UNSPSC Code: 41106514
• Biological source: African green monkey kidney
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Epithelial

Eigenschaften

• biological source: African green monkey kidney
• description: Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• packaging: tube of 5 μg 85102918-DNA-5UG, pkg of vial of cells 85102918-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• receptors: Not specified

Beschreibung

Biochem/physiol Actions

Established from an African green monkey foetal kidney. The cells are highly susceptible to Simian rotavirus SA11.

Please note: The species of origin of the MA104 cell line has been shown to be different from that claimed by the originators.

Whitaker & Hayward (1985) found the cell line to originate from an African green monkey and not a Rhesus macaque as was originally claimed. Whitaker AM and Hayward CJ (1985). The characterization of three monkey kidney cell lines. Develop. Biol. Standard. Vol 60: 125-131. PMID: 4043530. Abstract: Three monkey kidney cell lines, Vero, GL-V3 and MA-104 were subjected to karyological analysis to determine their chromosomal stability and to confirm their species of origin. Although the lines were shown to be relatively stable throughout all of the passage levels that were tested, the species of origin of one of them was found to be different from that claimed by the originators. This finding was supported by data from isoenzyme studies.

Monkey African Green kidney

Preparation Note

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 10% FBS / FCS (F2442).

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].