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M7145

MEM Non-essential Amino Acid Solution (100×)

Without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

MEM NEAA

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
eCl@ss:
32160406

Key Documents

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Product Name

MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture

Quality Level

200

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

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This Item
M4526M8042M2279
MEM Non-essential Amino Acid Solution (100×) without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

M7145

MEM Non-essential Amino Acid Solution (100×)

Sigma-Aldrich

Sigma-Aldrich

M4526

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M8042

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M2279

Minimum Essential Medium Eagle

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

400

Quality Level

400

Quality Level

400

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

General description

Eagle′s minimum essential medium (MEM) is a synthetic culture medium, which is composed of balanced salt solutions, and amino acids and vitamins. These components are essential for the growth of cultured cells. MEM might be supplemented with non-essential amino acids, to make MEM non-essential amino acid solution. The non-essential amino acids include L-alanine, L-asparagine, L-aspartic acid, L-glycine, L-serine, L-proline and L-glutamic acid.

Application

Adding supplements of amino acids to media both stimulates growth and prolongs the viability of cells in culture. MEM Non-essential Amino Acid Solution supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.
MEM Non-essential Amino Acid Solution has been used in Dulbecco′s minimum essential media for the maintenance of mammalian cells.[1] It has been supplemented in medium prepared for oocyte maturation.

Biochem/physiol Actions

Studies on hybridoma culture shows that addition of Eagle′s minimum essential medium (MEM) non-essential amino acid leads to enhanced membrane stability, during death phase of the culture. This also results in increased antibody secretion during death phase, and decreased lactate concentration.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Pricing

used together

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Pricing

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM1904
RNBM0563
RNBM0097
RNBL7298
RNBL4185

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Cell Culture
Jakoby WB
Methods in Enzymology, 58 (1979)
Influence of non-essential amino acids on apoptotic and necrotic death of mouse hybridoma cells in batch cultures
Fassnacht D
Biotechnology Letters, 19(1), 35-38 (1997)
Ayako Suzuki et al.
Genome biology, 16, 66-66 (2015-04-19)
To understand the heterogeneous behaviors of individual cancer cells, it is essential to investigate gene expression levels as well as their divergence between different individual cells. Recent advances in next-generation sequencing-related technologies have enabled us to conduct a single-cell RNA-Seq
Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization: relevance to intracellular reactive oxygen species and glutathione contents.
Hashimoto S et al.
Molecular Reproduction and Development, 56, 520-520 (2000)
Lei Xi Chen et al.
The Laryngoscope, 129(9), 2139-2146 (2019-03-19)
Surgical adhesives are increasingly used for vocal fold microsurgery to assist wound closure and reduce the risks of scar formation. Currently used vocal fold adhesives such as fibrin glue, however, have thus far not been found to promote wound closure
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Global Trade Item Number

SKUGTIN
M7145-100ML04061838259813
M7145-500ML04061837035951

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