108CC5

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: Mouse x Rat Hybridoma nerve
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Modal No. varied with passage see reference
• Morphology: Neuronal

Eigenschaften

• biological source: Mouse x Rat Hybridoma nerve
• packaging: tube of 5 μg 08062521-DNA-5UG, pkg of vial of cells 08062521-1VL
• growth mode: Adherent
• karyotype: Modal No. varied with passage see reference
• morphology: Neuronal
• products: Long processes after treatment with dibutyryl cAMP, dense core vesicles, excitable membranes, neurotransmitter enzymes, synthesis of neurohormones and uptake of catecholamines.
• receptors: Receptors for neurohormones: delta opioid, PGE 1 vasoactive intestinal polypeptide, adenosine, somatostatin and acetylcholine.
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Used as a neuronal Model

Biochem/physiol Actions

Mouse neuroblastoma x Rat glioma hybrid

The cell line108CC5 is formed by the fusion of mouse neuroblastoma N18TG2 and rat glioma C6-BU-1using inactivated Sendai virus. This cell line was renamed NG108-5 by Klee and Nirenberg . This is a cholinergic hybrid cell line.

Preparation Note

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.

Other Notes

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].