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C9409
Creatinine Deiminase microbial
lyophilized powder, ≥25 units/mg protein
Synonym(e):
Creatinine Deaminase
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 250 units | Warenkorb auf Verfügbarkeit prüfen | € 917,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-449-1
MDL number:
Specific activity:
≥25 units/mg protein
€ 917,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenform
lyophilized powder
Quality Level
specific activity
≥25 units/mg protein
mol wt
~260 kDa
composition
Protein, ≥15% biuret
storage temp.
−20°C
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| specific activity ≥25 units/mg protein | specific activity 100-300 units/mg protein | specific activity ≥4 units/mg solid | specific activity ≥150 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form salt-free, lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| mol wt ~260 kDa | mol wt ~175 kDa | mol wt ~100 kDa | mol wt 80-86.2 kDa |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| composition Protein, ≥15% biuret | composition Protein, 65-85% | composition - | composition - |
General description
Isoelectric point : 4.4
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)
Application
Creatinine Deiminase microbial has been used:
- to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine[1]
- in creating creatinine-sensing membrane for biophysical studies[2]
- to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples[3]
Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.
Biochem/physiol Actions
Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia.[1]
Physical form
Lyophilized powder containing mannitol as stabilizer
Other Notes
One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH3 per min at pH 7.5 at 37 °C in a coupled system with L-glutamic dehydrogenase.
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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R G Washburn et al.
The Journal of antimicrobial chemotherapy, 17(5), 673-677 (1986-05-01)
Creatinine iminohydrolase (EC 3.5.4.21) quantitatively releases ammonia from flucytosine (5FC) as well as from creatinine. Using 39 sera from eight patients receiving the combination of amphotericin B with 5FC, we demonstrated that this rapid enzymatic reaction provides a valid measure
Z K He et al.
Analytical biochemistry, 283(2), 166-174 (2000-07-25)
A precise and sensitive working microflow titration procedure was developed to determine creatinine and ammonia in urine samples. This procedure is based on enzymatic conversion of creatinine, gas diffusional membrane separation of the released ammonia into an acid acceptor stream
C S Rui et al.
Annals of the New York Academy of Sciences, 672, 264-271 (1992-11-30)
A flow-injection analysis biosensor system was developed for the amperometric assay of creatinine based on coupled reactions of three immobilized enzymes, using an oxygen electrode as the detection device. The ammonia produced by creatinine deiminase-catalyzed hydrolysis of creatinine was further
S M Colagiovanni et al.
Urology, 36(3), 283-285 (1990-09-01)
A patient with false elevation of serum creatinine level due to 5-fluorocytosine (5-FC) is reported. 5-FC interferes with the enzymatic method used for creatinine determination in the Kodak Ektachem analyzer. Clinicians should be aware of the potential for spurious azotemia
Manuel Gutiérrez et al.
Biosensors & bioelectronics, 23(6), 795-802 (2007-10-13)
Urea and creatinine biosensors based on urease and creatinine deiminase, respectively, covalently immobilized onto ammonium selective electrodes, were included in an array together with sensors sensitive to ammonium, potassium and sodium. Generic sensors to alkaline ions were also included. All
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C9409-250UN | 04061832755403 |
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