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C3172
Kreatininase aus Pseudomonas sp.
lyophilized powder, 100-300 units/mg protein
Synonym(e):
Creatinin-Amidohydrolase
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1000 UNITS
€ 430,00
About This Item
Empfohlene Produkte
Form
lyophilized powder
Spezifische Aktivität
100-300 units/mg protein
Mol-Gew.
~175 kDa
Zusammensetzung
Protein, 65-85%
Fremdaktivität
Creatinase and urease ≤1%
Hexokinase and ATPase ≤0.1%
Lagertemp.
2-8°C
Anwendung
Creatininase from microorganisms may be used in the preparation of amperometric biosensor by co-immobilization with other enzymes for the determination of creatinine.[1]
This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.
Biochem./physiol. Wirkung
Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.[2]
Physikalische Eigenschaften
Isoelectric point:4.7
Michaelis constants:3.2 x 10‾2M (Creatinine), 5.7 x 10‾2M (Creatine)
Structure:6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
Inhibitors:Ag+, Hg++, N-bromosuccinimide, EDTA
Optimum pH:6.5 − 7.5
Optimum temp:70°C
pH Stability:pH 7.5 − 9.0 (5°C, 16hr)
Thermal stability:Below 70°C (pH 7.5, 30 min)
Michaelis constants:3.2 x 10‾2M (Creatinine), 5.7 x 10‾2M (Creatine)
Structure:6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
Inhibitors:Ag+, Hg++, N-bromosuccinimide, EDTA
Optimum pH:6.5 − 7.5
Optimum temp:70°C
pH Stability:pH 7.5 − 9.0 (5°C, 16hr)
Thermal stability:Below 70°C (pH 7.5, 30 min)
Einheitendefinition
1 U hydrolysiert 1.0 μmol Creatinin zu Creatin pro Minute bei pH 8.0 und 25 °C
Physikalische Form
Lyophilisiertes Pulver, enthält Saccharose. ~70% des Proteins ist Rinderserumalbumin.
Hinweis zur Analyse
Proteingehalt mittels Biuret-Reaktion bestimmt.
Signalwort
Danger
H-Sätze
P-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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