C7774

Human Collagen Type I

from human placenta, powder, suitable for cell culture

• CAS-Nummer: 9007-34-5
• UNSPSC Code: 12352202
• NACRES: NA.61
• EC Number: 232-697-4
• MDL number: MFCD00130825
• Form: powder
• Biological source: human placenta

Eigenschaften

• Produktname: Kollagen aus menschlicher Placenta, Bornstein and Traub Type I (Sigma Type VIII), powder
• biological source: human placenta
• Quality Segment: 200
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: HIV, hepatitis B and hepatitis C, none detected
• UniProt accession no.: P02666, P02668, P53420, Q01955, Q14031
• storage temp.: 2-8°C
• Gene Information: bovine ... CSN2(281099), CSN3(281728) ; human ... COL4A3(1285), COL4A4(1286), COL4A6(1288)

Beschreibung

Application

This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm². It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

Biochem/physiol Actions

Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues.

Preparation Note

Prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.

Other Notes

Kollagen wird in eine Reihe an strukturell und genetisch verschiedenen Typen unterteilt. Wir nutzen die Nomenklatur, die von Bornstein und Traub vorgeschlagen wurde. Achten Sie darauf, dass Sie die Typenbezeichnungen von Sigma nicht mit anerkannten Typenklassifizierungen von Kollagen verwechseln.

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)