C7688

Chaperonin 60 from Escherichia coli

>95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder

• Synonym(e): Cpn60, GroEL
• UNSPSC Code: 12352200
• NACRES: NA.32
• MDL number: MFCD00163256
• Form: lyophilized powder
• Assay: >95% (SDS-PAGE)
• Biological source: Escherichia coli
• Recombinant: expressed in E. coli overproducing strain

Eigenschaften

• biological source: Escherichia coli
• Quality Segment: 200
• recombinant: expressed in E. coli overproducing strain
• assay: >95% (SDS-PAGE)
• form: lyophilized powder
• technique(s): electron microscopy: suitable, mass spectrometry (MS): suitable
• suitability: passes test (Refolding (w/GroES))
• UniProt accession no.: P10809
• storage temp.: 2-8°C
• Gene Information: human ... HSPD1(3329)

Beschreibung

General description

Research area: Cell Signaling

Chaperonin 60 (GroEL) and chaperonin 10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles.

Application

Chaperonin 60 from Escherichia coli has been used:

• in mass spectroscopy
• in cryo-electron microscopy imaging as control for testing particle distribution
• as standard in infrared spectrum measurements
• as a protein sample for stability assessment and characterization studies using differential mobility analysis (DMA) and cryo-electron microscopy (cryo-EM).

Biochem/physiol Actions

Apart from its role in facilitating protein folding, chaperonin 60 (Cpn60) serves as an extracellular signaling protein, showing functional similarities to certain proinflammatory cytokines. Furthermore, Cpn60 contributes to the inhibition of lipid accumulation and adipogenesis during the initial stages of cellular differentiation, thereby exerting anti-obesity effects.

Packaging

Package size based on protein content.

Physical form

Lyophilized powder containing Tris buffer salts, potassium chloride, magnesium chloride, dithiothreitol, and trehalose as stabilizer.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)