Fortfahren mit
C4478
S-Gal®/LB Agar-Mischung
reagent for selection of recombinant bacterial clones
Synonym(e):
Agar Blend
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Ansicht ändern
| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 500 mL | Warenkorb auf Verfügbarkeit prüfen | € 111,00 | |
| 6 x 500 mL | Warenkorb auf Verfügbarkeit prüfen | € 487,00 |
Über diesen Artikel
NACRES:
NA.85
UNSPSC Code:
41106200
€ 111,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltengrade
Molecular Biology
Quality Level
sterility
non-sterile
form
powder
technique(s)
microbiological culture: suitable
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
suitable for β-galactosidase test, nonselective for Escherichia coli, nonselective for coliforms
General description
S-Gal® (sodium salt) is an autoclavable, water-soluble, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Application
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® (sodium salt) is water-soluble, autoclavable and can be added to bacterial broth containing agar prior to autoclaving.
S-Gal®/LB Agar Blend has been used for the identification of recombinant bacterial colonies.[1]
Biochem/physiol Actions
When S-Gal® is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.
Features and Benefits
- More intense color contrast than X-gal
- Water-soluble and autoclavable for easiest use
- Convenient, pre-mixed media
Preparation Note
Suspend contents of one packet in 500 ml distilled or deionized water. Sterilize by autoclaving for 15 to 20 minutes at 121-124°C. For microwaving, heat suspended mix until initial boiling. Mix well. Heat for short intervals with mixing until agar component is in solution. Do not allow boiling for extended periods of time. Antibiotics should be added following autoclaving or microwaving, after cooling to 48-52°C.
Other Notes
Zusammensetzung (g/L):
Trypton, 10
Hefeextrakt, 5
Natriumchlorid, 10
Agar, 12
S-Gal, 0.3
Eisen(III)-ammonium-citrat, 0.5
IPTG, 0.03
Trypton, 10
Hefeextrakt, 5
Natriumchlorid, 10
Agar, 12
S-Gal, 0.3
Eisen(III)-ammonium-citrat, 0.5
IPTG, 0.03
The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
Legal Information
S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| suitability suitable for β-galactosidase test, nonselective for Escherichia coli, nonselective for coliforms | suitability suitable for substrate for β-galactosidase | suitability suitable for β-galactosidase test | suitability - |
| form powder | form powder | form powder | form powder |
| technique(s) microbiological culture: suitable | technique(s) nucleic acid detection: suitable | technique(s) nucleic acid detection: suitable | technique(s) microbiological culture: suitable |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| application(s) food and beverages | application(s) - | application(s) - | application(s) food and beverages |
| storage temp. room temp | storage temp. −20°C | storage temp. −20°C | storage temp. room temp |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Paola Jurado et al.
Molecular microbiology, 60(5), 1218-1227 (2006-05-13)
The identification of single-chain antibodies (scFvs) that interfere in vivo with the building of the complex that activate the prokaryotic, sigma54-dependent promoter Pu of the catabolic TOL plasmid pWW0 is reported. To this end, a phage M13 library of scFvs
LifeScience Quarterly: A superior dye to X-gal for clonal selection.
Heuermann, K. and Cosgrove, J.
Sigma data, 2(2), 2-4 (2001)
John Kelly et al.
Journal of bacteriology, 188(7), 2427-2434 (2006-03-21)
In eukaryotes, N-linked protein glycosylation is a universal modification involving addition of preformed oligosaccharides to select Asn-Xaa-Ser/Thr motifs and influencing multiple biological events. We recently demonstrated that Campylobacter jejuni is the first member of the Bacteria to possess an N-linked
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C4478-6X500ML | 04061833492222 |
| C4478-500ML | 04061837896682 |