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C2639

Cell Freezing Medium-Serum-free 1×

sterile-filtered, suitable for cell culture

Synonym(e):

Cryopreservation Medium, Serum-Free Freezing Medium

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PackungsgrößeSKUVerfügbarkeitPreis
50 mL
Warenkorb auf Verfügbarkeit prüfen
€ 247,00

Über diesen Artikel

NACRES:
NA.75
UNSPSC Code:
12352207

€ 247,00

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Unterstützung erhalten

sterility

sterile-filtered

Quality Segment

100

form

liquid

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

shipped in

ambient

storage temp.

2-8°C

Application

Sigma′s Cell Freezing Medium, Serum-free is a cryopreservation medium which does not contain DMSO or serum.
Cell Freezing Medium-Serum-free 1× has been used as a cryopreservation medium for somatic cell sampling and recovery (SCSR) exfoliated cells cultured in growth media and dihydrofolate reductase (DHFR)-deficient Chinese hamster ovary (CHO) cells grown in serum-free growth medium.[1]

Other Notes

Ready-to-use formula that does not contain dimethyl sulfoxide or serum.

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Dieser Artikel
C9249C6295C2999
Cell Freezing Medium-Serum-free 1× sterile-filtered, suitable for cell culture

Sigma-Aldrich

C2639

Cell Freezing Medium-Serum-free 1×

CryoSOfree™ DMSO-free Cryopreservation Medium

Sigma-Aldrich

C9249

CryoSOfree™ DMSO-free Cryopreservation Medium

Cell Freezing Medium-DMSO Serum free 1x sterile-filtered, suitable for cell culture

Sigma-Aldrich

C6295

Cell Freezing Medium-DMSO Serum free 1x

CryoStor® cell cryopreservation media CS5

Sigma-Aldrich

C2999

CryoStor® cell cryopreservation media

form

liquid

form

liquid

form

liquid

form

liquid

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

sterility

sterile-filtered

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

technique(s)

cell culture | mammalian: suitable, cryopreservation: suitable

shipped in

ambient

shipped in

wet ice

shipped in

dry ice

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

Lagerklasse

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
MKDB3490
MKCX7099
MKCW2551
MKCV5907
MKCT8013

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Artikel

Cell Culture Fundamentals: Cryopreservation and Storage of Cell Lines

Overview on liquid nitrogen storage and freezing of cell stocks for cell line cryopreservation purposes. Free ECACC handbook download.

Jeong Soo Park et al.
Protein expression and purification, 70(2), 231-235 (2009-10-13)
Hepatocyte growth factor (HGF) is a potent multi-functional protein that affects morphogenesis, cell migration, organ regeneration, and tumor invasion in various tissues, and has thus been considered to have potential as a therapeutic target in various diseases. In our current
Identification of a native novel oncolytic immunoglobulin on exfoliated colon epithelial cells: A bispecific heterodimeric chimera of IgA/IgG
Albaugh G P, et al.
Open Medicine : A Peer-Reviewed, Independent, Open-Access Journal, 10(6), 126-150 (2020)

Global Trade Item Number

SKUGTIN
C2639-50ML04061833484388

Questions

  1. Hi there, can i use this C2639 Cell Freezing Medium-Serum-free 1× for freezing HEK293 and MCF7 cells? In addition, can i use it to freeze hESC and hiPSC?

    1 answer

    1. This Cell Freezing Medium-Serum-free 1× does not contain dimethyl sulfoxide or serum and may not be suitable for all cell types. It is recommended to follow manufacturers' guidelines or literature for cell freezing. For example:

      - Human kidney (embryonic) 293 cells, Cat. #85120602-1VL: 5% dimethyl sulfoxide and 95% fetal calf serum.
      - MCF7 cells: 95% complete growth medium and 5% dimethyl sulfoxide.
      - Simplicon™ Human iPS cells: pluripotent media with 10% dimethyl sulfoxide.
      - Human embryonic stem cells: base medium with fetalbovine serum and 10% dimethyl sulfoxide.

      For cryopreservation of human embryonic stem cells or induced pluripotent stem cells in serum-free conditions, PluriSTEM™ Freeze Medium (SCM134) is recommended.

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