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Sigma-Aldrich

CryoSOfree™ DMSO-free Cryopreservation Medium

greener alternative

Synonym(e):

cell freezing medium

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75

Form

liquid

Qualitätsniveau

200

Grünere Alternativprodukt-Eigenschaften

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Methode(n)

cell culture | mammalian: suitable
cryopreservation: suitable

Grünere Alternativprodukt-Kategorie

, Aligned

Versandbedingung

wet ice

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

Cultured cells in a suspension can be cryopreserved with minimal damage from freezing and thawing steps. CryoSOfree™ DMSO-free cryopreservation medium preserves them with high viability and physiological activity. This medium is recommended for the preservation of stem cells, hepatocytes, tissue sample and a wide variety of cell types and established cell lines. CryoSOfree™ DMSO-free cryopreservation medium is a proprietary formulation, containing no DMSO nor animal proteins. It is ready-to-use and complete with no additives required.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of DMSO for cryopreservation of cell cultures. For more information see here.

Anwendung

CryoSOfree is a chemically defined DMSO-free cryopreservation medium. It is animal component free and has been shown to preserve the potential of stem cell for differentiation.

Leistungsmerkmale und Vorteile

  • Preserves pluripotency of stem cells
  • Low cytotoxicity
  • Chemically defined, serum free, protein free
  • Ready to use


The following cell lines have been recovered at ≥90% following cryopreservation with CryoSOfree:
L929, KB, MC3T3, B16F1, Colon26, CaCO2, HT1080, MG63

Angaben zur Herstellung

1 ml of CyroSOfree is sufficient for 5x105- 5x106 cells.

Rechtliche Hinweise

CryoSOfree is a trademark of Sigma-Aldrich Co. LLC

Piktogramme

Corrosion
GHS05

Signalwort

Warning

Gefahreneinstufungen

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Kazuaki Matsumura et al.
Cell transplantation, 19(6), 691-699 (2010-06-08)
Cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), glycerol, ethylene glycol, and propylene glycol have been used for the cryopreservation of cells and tissues. DMSO is the most effective CPA but shows high cytotoxicity and can effect differentiation. ɛ-poly-L-lysine (PLL)
Kazuaki Matsumura et al.
Biomaterials, 30(27), 4842-4849 (2009-06-12)
Dimethyl sulfoxide (DMSO) has been used for several decades as the most efficient cryoprotective agent (CPA) for many types of cells and tissues in spite of its cytotoxicity and its effects on differentiation. Here we report that polyampholytes with an
Matthew A Schaller et al.
JCI insight, 6(18) (2021-08-07)
Cell lines are the mainstay in understanding the biology of COVID-19 infection but do not recapitulate many of the complexities of human infection. The use of human lung tissue is one solution for the study of such novel respiratory pathogens.

Artikel

Animal Component Free Media & Reagents Frequently Asked Questions (FAQs)

Discover answers to your frequently asked questions about animal component-free, xenobiotic-free, and chemically defined media and reagents for cell culture.

Verwandter Inhalt

Herstellung von Zelltherapeutika

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Cell Therapy Manufacturing

Introducing a new cell therapy demands optimal performance in upstream and downstream processing, process development, testing, and manufacturing. World-class products and partners are critical to success, speeding advancement of therapeutics that are effective, safe, and affordable.

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