The complement is supplied in solution at a concentration of approximately 1 mg/mL, with the lot-specific certificate of analysis providing the exact concentration. Solubility is tested by diluting 0.1 mL of the complement stock solution with 0.2 mL of H2O, resulting in a final volume of 0.3 mL. This dilution ratio yields a complement concentration of approximately 0.33 mg/mL.
C1740
Komplementkomponente C1q aus Humanserum
≥95% (SDS-PAGE)
Synonym(e):
C1q from human serum
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0.5 MG
€ 505,00
1 MG
€ 885,00
Über diesen Artikel
Form:
liquid
Assay:
≥95% (SDS-PAGE)
Biological source:
human
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Dieser Artikel | |||
|---|---|---|---|
| technique(s) activity assay: suitable | technique(s) - | technique(s) - | technique(s) activity assay: suitable |
| assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay ≥90% (SDS-PAGE) |
| biological source human | biological source - | biological source - | biological source human serum |
| form liquid | form liquid | form liquid | form solution |
| shipped in dry ice | shipped in wet ice | shipped in wet ice | shipped in dry ice |
| Gene Information human ... C1QA(712), C1QB(713), C1QC(714) | Gene Information - | Gene Information - | Gene Information human ... C5(727) |
General description
C1q bildet zusammen mit C1r und C1s im Verhältnis 1:2:2 den C1-Komplex, der die erste Komponente des klassischen Komplementwegs darstellt. C1q besteht aus 18 Polypeptidketten (sechs A, sechs B, sechs C) (Molekulargewicht: 460 kDa). Alle enthalten einen Collagen-ähnlichen 81-aa-Bereich, der aus sich wiederholenden Sequenzen (Gly-Xaa-Yaa) nahe dem N-Terminus besteht. Drei Ketten (A1B1C1) bilden eine Dreifachhelix mit dem C-Terminus als globulären Kopf, der strukturell und funktionell unterschiedliche Domänen enthalten kann.
Application
Die Komplementkomponente C1q ist ein wichtiger Regulatorfaktor für die Plättchenaktivierung. Mit diesem Thema befasst sich die Forschung seit einiger Zeit, da Thrombozyten-Leukozyten-Aggregate bei inflammatorischen Erkrankungen, wie der koronaren Herzerkrankung, eine wichtige Rolle spielen. Insbesondere wurde nachgewiesen, dass C1q die Collagen-induzierte Aggregation hemmt und die Produktion von reaktiven Sauerstoffspezies (ROS) verbessert.
Biochem/physiol Actions
Eine C1q-Defizienz bedingt durch einen seltenen homozygoten Gendefekt oder transkriptionelle/translationale Defekte ist höchstwahrscheinlich eine Ursache des systemischen Lupus erythematodes (SLE). Die drei C1q-Untereinheiten werden durch einen neuartigen Mechanismus der transkriptionellen Synchronisation exprimiert.
Physical form
Wird als Lösung in 10 mM HEPES, 300 mM NaCl, pH 7,2, geliefert
Lagerklasse
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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The product is given at a concentration of approx. 1mg/ml. The product specification sheet says that product solubilized is clear, under conditions of 0.1 mL + 0.2 mL H2O. Is the concentration 1 mg/ml related to a volume of 0.1 mL (or 0.3 mL; or other?)?
1 answer-
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I ordered the C1740-1MG with lot#0000356664. I would like to know the protein sequences and the extinction coefficient about this protein?
1 answer-
This product corresponds to purified 'native' human C1q. Its sequence is not determined in-house. Please refer to published sequences, e.g. in GenBank for UniProt accession nos. P02745, P02746, & P02747.
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what is the size of the protein in WB ? when I test it's size it was approximately 24 KD ( the same size as c1q-c )
1 answer-
C1q is composed of 18 polypeptide chains (six A, six B, six C). When prepared in denaturing sample buffer, and run on SDS PAGE, the chains will likely dissociate and run at their individual molecular weights. The Uniprot entries for C1qA, C1qB, and C1qC list masses of 26,017, 26,722, and 25,774, respectively.
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Is this product suitable for use in cell cultures?
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This product has not been tested for suitability in cell culture. The end user will need to determine suitability. However, there are multiple publications citing the use of this product in cell culture applications. Please see the link below to review an example:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377823/Helpful?
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Hola, me gustaría saber como se reconstituye el producto para su uso en ensayos. Gracias.
1 answer-
This product is not suitable for use in clinical trials. Unless otherwise stated, this product is intended for research use only. It is not suitable for any other purpose, which includes, but is not limited to, unauthorized commercial use, in vitro diagnostic use, or therapeutic use in humans or animals.
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What is the concentration of C1740?
1 answer-
The product is supplied at a concentration of approximately 1 mg/ml. The exact concentration is lot specific and reported on the Certificate of Analysis.
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