C1655

Choleratoxin-B-Untereinheit

GM1-binding, lyophilized powder

• Synonym(e): CTB
• UNSPSC Code: 12352200
• PubChem Substance ID: 329774902
• NACRES: NA.77
• MDL number: MFCD02356522
• Form: lyophilized powder
• Quality level: 200

Eigenschaften

• Produktname: Choleratoxin-B-Untereinheit, FITC conjugate, lyophilized powder
• conjugate: FITC conjugate
• Quality Level: 200
• form: lyophilized powder
• mol wt: ~12 kDa
• composition: Protein, ~20% Lowry
• extent of labeling: ~1.0 mol FITC per mol protein
• storage temp.: 2-8°C
• SMILES string: CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F
• InChI: 1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
• InChI key: YDXZSNHARVUYNM-UHFFFAOYSA-N

Beschreibung

Application

Cholera Toxin B subunit-fluorescein isothiocyanate (FITC) conjugate has been used as a fluorescent tracer:

• to label distal cerebrospinal fluid-contacting neurons (dCSF-CNs) in lateral ventricles of rat[1]
• to stain epididymal sperms of mice[2]
• to stain peritoneal macrophages of mice[3]

Biochem/physiol Actions

Die Choleratoxin-B-Untereinheit wird in der neurologischen Forschung für das Track-Tracing verwendet; dies macht sich die GM1-Gangliosidbindung und den retrograden Transport zunutze.

Die Choleratoxin-B-Untereinheit wird in der neurologischen Forschung für das Track-Tracing verwendet; dies macht sich die GM1-Gangliosidbindung und den retrograden Transport zunutze. Mit Choleratoxin behandelte Gewebekulturzellen werden nicht abgetötet, und Gewebe von Tieren werden nicht nekrotisch.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide

Analysis Note

Activity measured by ELISA using ganglioside GM₁ coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H332,H412
• pcodes: P261 - P271 - P273 - P304 + P340 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Inhalation - Aquatic Chronic 3
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable