C9972

Choleratoxin-B-Untereinheit

mucosal immunogen, lyophilized powder

• Synonym(e): CTB
• UNSPSC Code: 12352200
• PubChem Substance ID: 329775297
• NACRES: NA.77
• MDL number: MFCD02356522
• Form: lyophilized powder
• Quality level: 200

Eigenschaften

• Produktname: Choleratoxin-B-Untereinheit, biotin conjugate, lyophilized powder
• conjugate: biotin conjugate
• Quality Level: 200
• form: lyophilized powder
• mol wt: ~12 kDa
• composition: Protein, ~40% Lowry
• storage temp.: 2-8°C
• SMILES string: CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F
• InChI: 1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
• InChI key: YDXZSNHARVUYNM-UHFFFAOYSA-N

Beschreibung

General description

Cholera Toxin B (CTB) is secreted by Vibrio cholerae. CTB functions as an oral subunit vaccine for cholera, which is associated with acute watery diarrhoea. It acts as a mucosal immunogen. Cholera toxin (CT) stimulates cell surface molecules, such as antigen presenting cells (APCs), murine and human dendritic cells (DCs). CT also has immunomodulatory properties. It induces the secretion of interleukin 1 (IL-1) from macrophages and enhances their APC function.

Application

Cholera Toxin B subunit has been used:

• in immunofluorescence[1]
• in the analysis of major histocompatibility complex (MHC) class II lipid raft partitioning
• in live cell three-dimensional tracking of SH-SY5Y human neuroblastoma cells
• to assess the toll-like receptors (TLR) and FcRγ (Fc receptor γ chain) – CARD9 (caspase recruitment domain family member 9) activation by cholera Toxin B (CTB)

Biochem/physiol Actions

Die Choleratoxin-B-Untereinheit wird in der neurologischen Forschung für das Track-Tracing verwendet; dies macht sich die GM1-Gangliosidbindung und den retrograden Transport zunutze.

Die Choleratoxin-B-Untereinheit wird in der neurologischen Forschung für das Track-Tracing verwendet; dies macht sich die GM1-Gangliosidbindung und den retrograden Transport zunutze. Mit Choleratoxin behandelte Gewebekulturzellen werden nicht abgetötet, und Gewebe von Tieren werden nicht nekrotisch.

Physical form

Lyophilized powder containing sodium phosphate buffer salts, sodium azide and sodium EDTA.

Analysis Note

Activity measured by ELISA using ganglioside GM₁-coated multiwell plates, rabbit anti-Cholera toxin B subunit, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding is achieved with 0.02-1 μg of Cholera toxin B subunit-biotin conjugate per mL. The conjugated B subunit gives a similar value for 50% binding to that of unconjugated B subunit from which it is prepared.

Biotin content ~1.0 mole/mole protein.

Sicherheitshinweise

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)