C1427

Cytochrome b₅ human

Name: Cytochrome b<SUB>5</SUB> human
Brand: SIGMA
Price: 569 EUR

recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)

Synonym(e):

Cytochrome b5

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0.1 MG

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Über diesen Artikel

UNSPSC Code:

12161501

NACRES:

NA.47

MDL number:

MFCD02687283

Form:

solution

Assay:

≥90% (SDS-PAGE)

Biological source:

human

Recombinant:

expressed in E. coli (histidine-tagged)

Mol wt:

16 kDa

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Unterstützung erhalten

biological source

human

recombinant

expressed in E. coli (histidine-tagged)

assay

≥90% (SDS-PAGE)

form

solution

mol wt

16 kDa

technique(s)

inhibition assay: suitable

suitability

suitable for molecular biology

UniProt accession no.

P00167

shipped in

dry ice

storage temp.

−70°C

Quality Level

200

Gene Information

human ... CYB5A(1528)

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Dieser Artikel	C3483	SRP0351	SRP0343
Sigma-Aldrich C1427 Cytochrome b₅ human	Sigma-Aldrich C3483 Cytochrome c from human heart	Sigma-Aldrich SRP0351 PDGFRb active human	Sigma-Aldrich SRP0343 cKit active human
technique(s) inhibition assay: suitable	technique(s) cell culture \| mammalian: suitable, mass spectrometry (MS): suitable, western blot (chemiluminescent): suitable	technique(s) activity assay: suitable, inhibition assay: suitable	technique(s) activity assay: suitable, inhibition assay: suitable
biological source human	biological source human heart	biological source human	biological source human
assay ≥90% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay ≥70% (SDS-PAGE)	assay ≥22% (SDS-PAGE)
recombinant expressed in E. coli (histidine-tagged)	recombinant -	recombinant expressed in baculovirus infected Sf9 cells	recombinant expressed in baculovirus infected Sf9 cells
form solution	form lyophilized powder	form aqueous solution	form aqueous solution
mol wt 16 kDa	mol wt 11 kDa by SDS-PAGE	mol wt 62 kDa	mol wt 53.5 kDa

General description

Cytochrome b5 (CYB5A) gene codes for cytochrome b5 protein.[1] It is located on human chromosome 18.[1] It is co-expressed in the Leydig cells of the testis and theca cells of the ovary.[2]

Biochem/physiol Actions

Cytochrome b5 (CYB5A) mediates NADH-dependent reduction of methemoglobin (erythrocytes), electron transfer to fatty-acid desaturases (mainly liver) and steroid and xenobiotics metabolism by cytochrome P450 enzymes.[1] It is essential for androstenedione synthesis. CYB5A helps in the efficient synthesis of gonadal androgen.[2]

Cytochrome b₅ is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown.

Membrane-bound heme-containing protein; enhances catalytic efficiency of some P450 isoforms.

Physical form

Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA

Analysis Note

Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM^-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b₅.

Lagerklasse

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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The sphingosine 1-phosphate receptor 2/4 antagonist JTE-013 elicits off-target effects on sphingolipid metabolism.

Melissa R Pitman et al.

Scientific reports, 12(1), 454-454 (2022-01-12)

Sphingosine 1-phosphate (S1P) is a signaling lipid that has broad roles, working either intracellularly through various protein targets, or extracellularly via a family of five G-protein coupled receptors. Agents that selectively and specifically target each of the S1P receptors have

CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women

Stark K, et al.

Arthritis Research & Therapy, 17(1), 56-56 (2015)

Human adrenal cells that express both beta-hydroxysteroid dehydrogenase type 2 (HSD3B2) and cytochrome b5 (CYB5A) contribute to adrenal androstenedione production

Nakamura Y, et al.

The Journal of Steroid Biochemistry and Molecular Biology, 123(3-5), 122-126 (2011)

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Cytochrome b5 human