This recombinant protein is full-length and includes the transmembrane domain.
Fortfahren mit
C1427
Cytochrome b5 human
recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)
Synonym(e):
Cytochrome b5
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Ansicht ändern
| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 0.1 mg | Warenkorb auf Verfügbarkeit prüfen | € 569,00 |
Über diesen Artikel
Form:
solution
Assay:
≥90% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli (histidine-tagged)
Mol wt:
16 kDa
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenbiological source
human
Quality Segment
recombinant
expressed in E. coli (histidine-tagged)
assay
≥90% (SDS-PAGE)
form
solution
mol wt
16 kDa
technique(s)
inhibition assay: suitable
suitability
suitable for molecular biology
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CYB5A(1528)
General description
Cytochrome b5 (CYB5A) gene codes for cytochrome b5 protein. It is located on human chromosome 18. It is co-expressed in the Leydig cells of the testis and theca cells of the ovary.
Biochem/physiol Actions
Cytochrome b5 is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown.
Membrane-bound heme-containing protein; enhances catalytic efficiency of some P450 isoforms.
Cytochrome b5 (CYB5A) mediates NADH-dependent reduction of methemoglobin (erythrocytes), electron transfer to fatty-acid desaturases (mainly liver) and steroid and xenobiotics metabolism by cytochrome P450 enzymes. It is essential for androstenedione synthesis. CYB5A helps in the efficient synthesis of gonadal androgen.
Physical form
Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA
Analysis Note
Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b5.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| technique(s) inhibition assay: suitable | technique(s) electrophoretic mobility shift assay: suitable | technique(s) activity assay: suitable, inhibition assay: suitable | technique(s) activity assay: suitable, inhibition assay: suitable |
| biological source human | biological source human | biological source human | biological source human |
| assay ≥90% (SDS-PAGE) | assay ≥80% (SDS-PAGE) | assay ≥22% (SDS-PAGE) | assay ≥60% (SDS-PAGE) |
| recombinant expressed in E. coli (histidine-tagged) | recombinant expressed in E. coli | recombinant expressed in baculovirus infected Sf9 cells | recombinant expressed in baculovirus infected Sf9 cells |
| suitability suitable for molecular biology | suitability - | suitability - | suitability - |
| form solution | form frozen liquid | form aqueous solution | form aqueous solution |
Lagerklasse
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C1427-.1MG | 04061833467589 |
-
does this recombinant version of the protein still contain its membrane bound domain?
1 answer-
Helpful?
-