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C1224

Sigma-Aldrich

Caspase 3 human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, ≥1.0 units/mg protein

Synonym(e):

Apopain, CPP32, Yama

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About This Item

EC-Nummer:
3.4.22.56 (BRENDA, IUBMB)
MDL-Nummer:
MFCD01634593
UNSPSC-Code:
12352204
NACRES:
NA.32

Rekombinant

expressed in E. coli (C-terminal histidine-tagged)

Qualitätsniveau

200

Assay

≥90% (SDS-PAGE)

Form

buffered aqueous glycerol solution

Spezifische Aktivität

≥1.0 units/mg protein

Mol-Gew.

~30 kDa

UniProt-Hinterlegungsnummer

P42574

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... CASP3(836)

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Allgemeine Beschreibung

Research area: Apoptosis

Human recombinant C-terminal histidine tagged caspase 3 is a fully active protein consisting of 17 kDa and 13.5 kDa subunits; the 13.5 kDa subunit contains the histidine tag.

Anwendung

Caspase 3 human has been used to digest human r-vimentin. It has also been used in caspase-3 inhibition assay and protease inhibition assay.

This product may also be used in cell-based apoptosis assay.

Biochem./physiol. Wirkung

Caspase 3 is a member of the CED-3 subfamily of caspases and is responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation. Caspase 3 is considered to be an effector caspase, activating pro-caspase 6 and pro-caspase 9 in vitro. Caspase 3 can be activated by caspase 8, caspase 6, and granzyme B.

Einheitendefinition

One unit will cleave 1.0 μmole of N-acetyl-Asp-Glu-Val-Asp-pNA per min at pH 7.4 at 25 °C.

Physikalische Form

Solution in 10% (w/v) glycerol containing 50 mM HEPES, pH 7.4, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS

Angaben zur Herstellung

Caspase 3 is synthesized as a 32 kDa proenzyme. The active enzyme is a heterodimer of two large (17 kDa) subunits and two small (12 kDa) subunits.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

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Die Dokumentenbibliothek aufrufen

A peptide-based positron emission tomography probe for in vivo detection of caspase activity in apoptotic cells
Hight MR, et al.
Clinical Cancer Research, 20(8), 2126-2135 (2014)
Cross-presentation of caspase-cleaved apoptotic self antigens in HIV infection
Rawson PM, et al.
Nature Medicine, 13(12), 1431-1431 (2007)
Deju Ye et al.
Nature chemistry, 6(6), 519-526 (2014-05-23)
Directed self-assembly of small molecules in living systems could enable a myriad of applications in biology and medicine, and already this has been used widely to synthesize supramolecules and nano/microstructures in solution and in living cells. However, controlling the self-assembly
Petra Procházková et al.
PloS one, 9(10), e109900-e109900 (2014-10-04)
Iron homeostasis in cells is regulated by iron regulatory proteins (IRPs) that exist in different organisms. IRPs are cytosolic proteins that bind to iron-responsive elements (IREs) of the 5'- or 3'-untranslated regions (UTR) of mRNAs that encode many proteins involved
Regulation of spermatogenic cell apoptosis by the pro-apoptotic proteins in the testicular tissues of mammalian and avian species
Zakariah M, et al.
Animal Reproduction Science (2022)
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