B5525

Bromphenolblau Natriumsalz

• Synonym(e): 3′,3′′,5′,5′′-Tetrabrom-phenolsulfophthalein Natriumsalz
• Empirische Formel (Hill-System): C₁₉H₉Br₄NaO₅S
• CAS-Nummer: 34725-61-6
• Molekulargewicht: 691.94
• UNSPSC Code: 12171500
• NACRES: NA.52
• PubChem Substance ID: 57653941
• EC Number: 252-170-2
• Beilstein/REAXYS Number: 6995701
• MDL number: MFCD00013793

Eigenschaften

• form: powder
• Quality Segment: 200
• technique(s): electrophoresis: suitable
• solubility: water: soluble
• suitability: suitable for nucleic acid electrophoresis
• storage temp.: room temp
• SMILES string: [Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O
• InChI: 1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1
• InChI key: TWKDJMRHSLWRFI-UHFFFAOYSA-M

Beschreibung

General description

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Ein Tracking-Farbstoff für Nuklein- oder Proteinelektrophorese in Agarose- oder Polyacrylamidgels.

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis.[1] It has also been used in Drosphila fly food to aid visualization of gut for dissection.[2]

Preparation Note

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)