This product is quite stable and is not assigned an expiration or retest date. A one-year warranty from the date of shipment applies. Provided that the product is handled appropriately, securely sealed, and not contaminated, the material is stable for at least the 1 year warranty period, both opened and unopened. Please see the link below to review the full Product Dating Information Statement:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/356/113/product-dating-information-06-25-ms.pdf
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A8582
Antifoam SE-15
aqueous emulsion for bacterial and mammalian systems
Synonym(e):
silicone-based antifoam
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 100 g | Warenkorb auf Verfügbarkeit prüfen | € 54,80 | |
| 500 g | Warenkorb auf Verfügbarkeit prüfen | € 200,00 |
Über diesen Artikel
NACRES:
NA.85
UNSPSC Code:
12161901
Technischer Dienst
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Unterstützung erhaltenQuality Segment
form
emulsion (aqueous)
concentration
10% (active silicon)
technique(s)
cell culture | hybridoma: suitable
solubility
H2O: soluble (effective hot or cold.)
density
1.0 g/mL at 25 °C
application(s)
microbiology
storage temp.
room temp
suitability
(Mammalian (suspension)), bacteria (fermentation)
General description
Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.
Application
Antifoam SE-15 has been used:
- in the preparation of ramified rolling circle amplification (RAM) amplification buffer.
- to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.
- as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay. [1]
- as a component of autoinduction expression culture. [2]
- as a component of cyclic adenosine monophosphate (cAMP) detection buffer. [3]
Features and Benefits
- Controls foaming in microbial culture flasks or fermentations
- Non-toxic to the culture medium
- Can be pumped to fermenter on an as-needed basis
Preparation Note
Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted
suspensions should be used immediately.
suspensions should be used immediately.
Other Notes
Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .
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Dieser Artikel | |||
|---|---|---|---|
| form emulsion (aqueous) | form emulsion (aqueous) | form emulsion (aqueous) | form emulsion |
| technique(s) cell culture | hybridoma: suitable | technique(s) cell culture | hybridoma: suitable, microbiological culture: suitable | technique(s) - | technique(s) cell culture | hybridoma: suitable, microbiological culture: suitable |
| suitability (Mammalian (suspension)), bacteria (fermentation) | suitability (Mammalian (suspension)), bacteria (fermentation) | suitability (Mammalian (suspension)), bacteria (fermentation) | suitability - |
| application(s) microbiology | application(s) microbiology | application(s) microbiology | application(s) - |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. - |
| solubility H2O: soluble (effective hot or cold.) | solubility - | solubility - | solubility - |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - STOT RE 2 Inhalation
target_organs
Lungs
Lagerklasse
12 - Non Combustible Liquids
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Verwandter Inhalt
Detection of nucleic acid targets using ramified rolling circle DNA amplification: a single nucleotide polymorphism assay model
Smith J H and Beals T P
PLoS ONE, 8(5), e65053-e65053 (2013)
Dario A Vitturi et al.
American journal of physiology. Heart and circulatory physiology, 296(5), H1398-H1407 (2009-03-17)
Allosteric regulation of nitrite reduction by deoxyhemoglobin has been proposed to mediate nitric oxide (NO) formation during hypoxia. Nitrite is predominantly an anion at physiological pH, raising questions about the mechanism by which it enters the red blood cell (RBC)
James H Smith et al.
PloS one, 8(5), e65053-e65053 (2013-06-01)
Isothermal amplification methods provide alternatives to PCR that may be preferable for some nucleic acid target detection tasks. Among current isothermal target detection methods, ramified rolling circle amplification (RAM) of single-stranded DNA circles that are formed by ligation of linear
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A8582-100G | 04061833392287 |
| A8582-500G | 04061835572038 |
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What is the shelf-life of product A8582 unopened as well as after the bottle is opened and first used? Is there a dependency of that shelf-life on the date of manufacture? And where can the customer access that information?
1 answer-
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細胞培養液に添加するためにアンチフォームSE-15の購入を検討しております。この商品は、オートクレーブ可能でしょうか、オートクレーブ可能の場合何回程度が限度でしょうか。 もし可能であれば、アンチフォーム C エマルジョンとの違いも教えていただけないでしょうか。 （アンチフォーム C エマルジョンもオートクレーブ可能でしょうか。） よろしくお願い申し上げます。
1 answer-
Antifoam SE-15 is a 10% emulsion of active silicone polymer and non-ionic emulsifiers. It can be repeatedly sterilized by autoclaving. Antifoam C Emulsion is an aqueous emulsion containing 30% active silicone. Further details about the different properties can be found in the datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/316/313/a8582pis.pdfHelpful?
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At what concentration is SE-15 (A8582) to be used in bacterial cell culture to control foam?
1 answer-
This Antifoam product is typically used at starting concentrations of 0.01 - 0.02%. Optimization may be required based on variable culture density, ingredients, and conditions. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/133/175/a8582dat.pdfHelpful?
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I was wondering if you had any information in regards to the stability of antifoam SE-15, A8582. In particular, do you have any data about the stability of this product at pH 8.0? When in a solution, do you have any data on the product stability?
1 answer-
This product is stable at room temperature. Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted suspensions should be used immediately. The stability of this product is not tested at pH 8.0. If you are looking for antifoams that will be stable at pH 8.0, perhaps you may consider Antifoam A Concentrate (Product A5633 and A6582), They are stable at pH 5.0-9.0. Please see this link for any additional information on Product A8582: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/316/313/a8582pis.pdf
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