A5598

Anti-cMyc in Kaninchen hergestellte Antikörper

Name: Anti-cMyc in Kaninchen hergestellte Antikörper
Brand: SIGMA
Price: 652.8 EUR

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Anti-c-Myc antibody produced in rabbit

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500 μG

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Über diesen Artikel

UNSPSC Code:

12352203

NACRES:

NA.56

MDL number:

MFCD00239651

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

Citations:

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Unterstützung erhalten

biological source

rabbit

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

concentration

1.8-4 mg/mL

technique(s)

western blot: 1:5,000

UniProt accession no.

P01106

storage temp.

−20°C

Quality Level

200

Gene Information

human ... MYC(4609)

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Dieser Artikel	C3956	SAB4503660	B7554
Sigma-Aldrich A5598 Anti-c-Myc−Peroxidase antibody produced in rabbit	Sigma-Aldrich C3956 Anti-c-Myc antibody produced in rabbit	Sigma-Aldrich SAB4503660 Anti-MYC antibody produced in rabbit	Sigma-Aldrich B7554 Anti-c-Myc−Biotin antibody, Mouse monoclonal
biological source rabbit	biological source rabbit	biological source rabbit	biological source mouse
conjugate peroxidase conjugate	conjugate unconjugated	conjugate unconjugated	conjugate biotin conjugate
clone polyclonal	clone polyclonal	clone polyclonal	clone 9E10, monoclonal
species reactivity human	species reactivity human	species reactivity human, mouse, rat	species reactivity human
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture
technique(s) western blot: 1:5,000	technique(s) immunocytochemistry: 5-10 μg/mL, immunoprecipitation (IP): 1-2 μg using immunoprecipitates a c-Myc fusion protein from transfected mammalian cell lysates or bacterial extracts, indirect immunofluorescence: 5-10 μg/mL using detects c-Myc fusion proteins in methanol-acetone fixed transiently transfected cells, microarray: suitable, western blot: 0.5-1 μg/mL using detects c-Myc fusion proteins in cell extracts from transfected cultures as well as bacterial lysates.	technique(s) ELISA: 1:40000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000	technique(s) western blot (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells or E. coli cells expressing c-Myc tagged fusion protein

General description

The Anti-c-Myc peroxidase conjugate rabbit antibody reacts with c-Myc tagged fusion proteins. It recognizes the c-Myc tag sequence expressed at either the amino- or the carboxyl-terminus.

Immunogen

Peptid entsprechend den Aminosäuren 408–425 des humanen c-myc-Proto-Onkogens, über einen C-terminalen hinzugefügten Cysteinrest an Maleimid-aktiviertes KLH konjugiert.

Application

Antibody is suitable for immunoblotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

N-terminal oder C-terminal markierte (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) c-Myc-Fusionsproteine.

Physical form

Solution in 0.01 M sodium phosphate buffer saline, pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

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pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

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Efg1-mediated recruitment of NuA4 to promoters is required for hypha-specific Swi/Snf binding and activation in Candida albicans.

Yang Lu et al.

Molecular biology of the cell, 19(10), 4260-4272 (2008-08-08)

Efg1 is essential for hyphal development and virulence in the human pathogenic fungus Candida albicans. How Efg1 regulates gene expression is unknown. Here, we show that Efg1 interacts with components of the nucleosome acetyltransferase of H4 (NuA4) histone acetyltransferase (HAT)

Du-Hwa Lee et al.

The Plant cell, 29(11), 2895-2920 (2017-11-01)

Dynamic control of protein translation in response to the environment is essential for the survival of plant cells. Target of rapamycin (TOR) coordinates protein synthesis with cellular energy/nutrient availability through transcriptional modulation and phosphorylation of the translation machinery. However, mechanisms

The Arabidopsis O-fucosyltransferase SPINDLY activates nuclear growth repressor DELLA.

Rodolfo Zentella et al.

Nature chemical biology, 13(5), 479-485 (2017-03-01)

Plant development requires coordination among complex signaling networks to enhance the plant's adaptation to changing environments. DELLAs, transcription regulators originally identified as repressors of phytohormone gibberellin signaling, play a central role in integrating multiple signaling activities via direct protein interactions

Coordinated transcriptional regulation underlying the circadian clock in Arabidopsis.

Gang Li et al.

Nature cell biology, 13(5), 616-622 (2011-04-19)

The circadian clock controls many metabolic, developmental and physiological processes in a time-of-day-specific manner in both plants and animals. The photoreceptors involved in the perception of light and entrainment of the circadian clock have been well characterized in plants. However

Bacterial E3 Ubiquitin Ligase IpaH4.5 of Shigella flexneri Targets TBK1 To Dampen the Host Antibacterial Response.

Zirui Zheng et al.

Journal of immunology (Baltimore, Md. : 1950), 196(3), 1199-1208 (2015-12-25)

IFN regulatory factors play a pivotal role in many cellular processes, including inflammatory and immune responses. Their activation is tightly regulated by TANK-binding kinase 1 (TBK1). In response to microbial components, TBK1 activates IFN regulatory factor 3 (IRF3) and cytokine

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