This product is a crude enzyme preparation, the activity is not a determined test specification. Historical data indicates activity may range from 10 - 40 units per gram. Please see the link below to review the available assay protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/192/355/a5384enz.pdf
Fortfahren mit
A5384
Aconitase from porcine heart
Synonym(e):
Aconitate hydratase, Citrate (isocitrate) hydrolyase
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 150 mg | Warenkorb auf Verfügbarkeit prüfen | € 389,00 | |
| 250 mg | Warenkorb auf Verfügbarkeit prüfen | € 562,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Biological source:
Porcine heart
€ 389,00
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Technischer Dienst
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Unterstützung erhaltenbiological source
Porcine heart
Quality Level
form
solid
mol wt
~66 kDa by gel filtration
storage temp.
−20°C
Application
Biochem/physiol Actions
Aconitase catalyses the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle. Aconitase is inhibited by cyanide, sulfide and copper and mercury at low concentrations. It is competitively inhibited by trans-aconitate. Aconitase contains an internal Fe-S cluster which is responsible for its activity.
Analysis Note
Crude preparation containing aconitase activity
Other Notes
One unit will convert 1.0 μmole of citrate (via cis-aconitate) to isocitrate per min at pH 7.4 at 25 °C.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| biological source Porcine heart | biological source Porcine heart | biological source Porcine heart | biological source Porcine liver |
| form solid | form solid | form liquid | form ammonium sulfate suspension |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt ~66 kDa by gel filtration | mol wt - | mol wt - | mol wt 168 kDa |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Verwandter Inhalt
Product Information Sheet
QC Methods
Ewelina Michta et al.
Environmental microbiology, 14(12), 3203-3219 (2012-11-03)
In many organisms, aconitases have dual functions; they serve as enzymes in the tricarboxylic acid cycle and as regulators of iron metabolism. In this study we defined the role of the aconitase AcnA in Streptomyces viridochromogenes Tü494, the producer of
Megan J Morgan et al.
Plant physiology, 161(1), 397-407 (2012-11-21)
Organic acid content is regarded as one of the most important quality traits of fresh tomato (Solanum lycopersicum). However, the complexity of carboxylic acid metabolism and storage means that it is difficult to predict the best way to engineer altered
Lucy M Hinder et al.
The Journal of endocrinology, 216(1), 1-11 (2012-10-23)
Diabetic neuropathy (DN) is the most common complication of diabetes and is characterized by distal-to-proximal loss of peripheral nerve axons. The idea of tissue-specific pathological alterations in energy metabolism in diabetic complications-prone tissues is emerging. Altered nerve metabolism in type
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A5384-250MG | 04061833423905 |
| A5384-150MG | 04061833616352 |
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