A2554

Anti-Maus-IgG (Fc-spezifische)-Peroxidase in Ziege hergestellte Antikörper

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Goat Anti-Mouse IgG (Fc specific)–HRP
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162629
• Conjugate: peroxidase conjugate
• Clone: polyclonal
• Application: ELISA (d), IHC (p), WB CL
• Citations: 167

Eigenschaften

• biological source: goat
• Quality Segment: 300
• conjugate: peroxidase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: mouse
• should not react with: bovine, human, horse
• technique(s): direct ELISA: 1:70,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:80,000-1:160,000 using total cell extract of Hela cells (5-10 μg/mL)
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Beschreibung

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Each immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Application

Die Spezifität eines neuen Antikörpers für PND im Blut immunisierter Mäuse wurde mit einem ELISA unter Verwendung von mit biotinyliertem PND und Streptavidin beschichteten Platten getestet. HRP-konjugierte Anti-Maus-IgG-Antikörper von Ziegen (Fc-spezifisch) wurden als Sekundärmittel verwendet.

Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat has been used in:

• immunohistochemistry
• enzyme-linked immunosorbent assay (ELISA)
• immunoblot
• dot-blot analysis

Biochem/physiol Actions

Goat anti-mouse IgG(Fc specific)-peroxidase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The conjugate shows no reactivity with mouse Fab fragment, human IgG, IgA, IgM, κ and λ light chain, bovine IgG and IgM, or horse IgG.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization and antibody dependent cell mediated cytotoxicity.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Adsorbed to reduce background staining with bovine, horse, or human samples.

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Antibody adsorbed with bovine, equine and human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable