The typical final working concentrations for cell loading is in the range of 1 to 10 micromolar. It is best to find literature references dealing with your application for more information on the amounts to use.
A1076
BAPTA-AM
≥95% (HPLC), powder, intracellular Ca²⁺ store chelator
Synonym(e):
1,2-Bis(2-aminophenoxy)ethan-N,N,N′,N′-tetraessigsäure-tetrakis(acetoxymethylester)
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Über diesen Artikel
Empirische Formel (Hill-System):
C34H40N2O18
CAS-Nummer:
Molekulargewicht:
764.68
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
Beilstein/REAXYS Number:
7458534
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Unterstützung erhaltenProduktname
BAPTA-AM, ≥95% (HPLC)
Quality Level
assay
≥95% (HPLC)
form
powder
storage temp.
−20°C
SMILES string
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccccc1OCCOc2ccccc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O
InChI
1S/C34H40N2O18/c1-23(37)47-19-51-31(41)15-35(16-32(42)52-20-48-24(2)38)27-9-5-7-11-29(27)45-13-14-46-30-12-8-6-10-28(30)36(17-33(43)53-21-49-25(3)39)18-34(44)54-22-50-26(4)40/h5-12H,13-22H2,1-4H3
InChI key
YJIYWYAMZFVECX-UHFFFAOYSA-N
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Verwandte Kategorien
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| form powder | form solid | form solid | form - |
| assay ≥95% (HPLC) | assay ≥90% (TLC) | assay - | assay ≥90% (HPLC) |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
BAPTA-AM hemmt die von freien Radikalen vermittelte Toxizität, fördert die Apoptose bei nicht-neuronalen Zellen und schützt Nervenzellen vor ischämischem Schaden. Es reguliert die Ionenkanäle und blockiert neuronale, Ca2+-aktivierte K+ Kanalströme. BAPTA-AM wahrt die intrazelluläre Ca2+-Homöostase.
Application
BAPTA-AM hat folgende Anwendungen:
- für die Verringerung der Erhöhung von gespaltener Caspase-3
- für die Blockierung des Zelltodes, Hemmung der Produktion von reaktiven Sauerstoffspezies (ROS) und Hemmung der Aktivierung von Caspase-8
- für das Ausschließen der Auswirkungen von Veränderungen der intrazellulären Ca2+ ([Ca2+]i)-Konzentration bei der Induktion von Krampfanfällen
- zur Verhinderung einer Zunahme von [Ca2+]i, induziert durch 6-Hydroxydopamin (6-OHDA)
Biochem/physiol Actions
Selektiver Chelator von intrazellulären Ca2+-Speichern.
Other Notes
Membranpermeable Form von BAPTA.
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells
Tang Q, et al.
Biochemical Pharmacology, 74(11), 1596-1607 (2007)
Glycolysis is essential for chemoresistance induced by transient receptor potential channel C5 in colorectal cancer
Wang T, et al.
BMC Cancer, 18(1), 207-207 (2018)
Hyperactivation of the mammalian degenerin MDEG promotes caspase-8 activation and apoptosis
Pan JA, et al.
The Journal of Biological Chemistry, 288(5), 2952-2963 (2013)
Blockade of RyRs in the ER Attenuates 6-OHDA-induced calcium overload, cellular hypo-excitability and apoptosis in dopaminergic neurons
Huang L, et al.
Frontiers in Cellular Neuroscience, 11, 52-52 (2017)
M-calpain activation facilitates seizure induced KCC2 down regulation
Wan L, et al.
Frontiers in Molecular Neuroscience, 11, 287-287 (2018)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| A1076-25MG | 04061833334867 |
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What is the typical working concentration for BAPTA-AM, Product A1076?
1 answer-
Helpful?
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How would you recommend that I store my BAPTA-AM, Product A1076, solution?
1 answer-
Stock solutions of 1-10 mM can be prepared in anhydrous DMSO, divided into single-use aliquots, and stored desiccated and frozen at -20°C.
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In what solvents is BAPTA-AM, Product A1076, soluble?
1 answer-
BAPTA-AM is soluble in dimethyl sulfoxide (DMSO) at 25 mg/mL. It is also soluble in acetonitrile at 0.5 mg/mL. It is best to use anhydrous DMSO, since it is known that moisture uptake by the DMSO can adversely affect the quality of the BAPTA-AM.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What are the shipping and storage temperatures for BAPTA-AM, Product A1076?
1 answer-
BAPTA-AM is shipped at ambient temperature. The recommended storage temperature, once received, is -20 °C.
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What is BAPTA-AM, Product A1076, and how is it used?
1 answer-
BAPTA-AM is a cell-permeant acetoxymethyl ester derivative of BAPTA (Product No. A4926), which is used as a chelator of intracellular Ca2+ stores. The acetoxymethyl ester derivative, which is an ester of the carboxylic acid groups in BAPTA, results in an uncharged molecule which allows it to penetrate cell membranes. Inside the cell, these groups are cleaved by nonspecific esterase activity resulting in the free acid form.
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