This product is a complex that has an estimated molecular weight of 422.5 g/mole. The solubility at 400 mM has not been tested. This product is offered in a prepared solution under product 94742 at a concentration of 200 mM. Based on the estimated molecular weight, a 0.4 M (400 mM) solution would have a concentration of 169 mg/mL. Solutions may be prepared in nuclease-free PBS containing 15% glycerol or 5% DMSO as a cryoprotectant. Solutions may then be stored in working aliquots at −20˚C. Avoid the use of EDTA in all buffer solutions as this will cause degradation of the complex. See the link below to publication that may be helpful. http://diyhpl.us/~bryan/irc/protocol-online/protocol-cache/view_cache.cgi%3FID=3486
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94740
Vanadyl-Ribonukleosid-Komplexe
powder
Synonym(e):
Ribonucleosid-Vanadyl-Komplexe, VRC
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 250 mg | Warenkorb auf Verfügbarkeit prüfen | € 124,00 | |
| 1 g | Warenkorb auf Verfügbarkeit prüfen | € 397,00 |
Über diesen Artikel
UNSPSC Code:
12352200
NACRES:
NA.52
Form:
powder
Storage temp.:
−20°C
€ 124,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenProduktname
Vanadyl-Ribonukleosid-Komplexe, BioReagent, Molecular Biology
grade
Molecular Biology
Quality Level
product line
BioReagent
form
powder
suitability
in accordance for ribonuclease inhibition test
shipped in
wet ice
storage temp.
−20°C
General description
Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.[1]
Application
Vanadyl ribonucleoside complexes has been used in hybridization mixture for RNA in situ hybridization.
Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.
Biochem/physiol Actions
The vanadyl ribonucleoside complex has the ability to block the formation of ribosomal subunit in Staphylococcus aureus. The complex exhibits anti-microbial activity[1] These complexes also serve as powerful inhibitors of several enzymes with ribonucleolytic activity.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| grade Molecular Biology | grade Molecular Biology | grade - | grade - |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| form powder | form liquid | form liquid | form powder |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in ambient |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| suitability in accordance for ribonuclease inhibition test | suitability in accordance for ribonuclease inhibition test | suitability - | suitability - |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Analysis of TMEM174 gene expression in various renal cancer types by RNA in situ hybridization
Zhang X, et al.
Oncology Letters, 8(4), 1693-1696 (2014)
Isolation of cytoplasmic RNA: ribonucleoside-vanadyl complexes.
S L Berger
Methods in enzymology, 152, 227-234 (1987-01-01)
R Boom et al.
Journal of clinical microbiology, 28(3), 495-503 (1990-03-01)
We have developed a simple, rapid, and reliable protocol for the small-scale purification of DNA and RNA from, e.g., human serum and urine. The method is based on the lysing and nuclease-inactivating properties of the chaotropic agent guanidinium thiocyanate together
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 94740-1G | 04061833296530 |
| 94740-250MG | 04061833296547 |
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Can you provide me with a protocol for preparing a solution of Vanadyl-ribonucleoside complex (94740-1G) at a concentration of 400 mM?
Can you provide me with a protocol for preparing a solution of Vanadyl-ribonucleoside complex (94740-1G) at a concentration of 400 mM?
1 answer-
Helpful?
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How to make 200 mM from 94740-250MG vanadyl ribonucleoside complexes powder?
1 answer-
The method to prepare a 200mM solution of the 94740 is proprietary and involves in-process testing. However, product 94742 and R3380 are prepared 200mM solutions of Vanadyl Ribonucleoside complexes.
Helpful?
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