79636

Atto 590-NHS-ester

BioReagent, suitable for fluorescence, ≥90.0% (degree of coupling)

• Empirische Formel (Hill-System): C₄₁H₄₂ClN₃O₁₁
• CAS-Nummer: 670269-33-7
• Molekulargewicht: 788.24
• UNSPSC Code: 12352108
• NACRES: NA.32
• MDL number: MFCD03456133

Eigenschaften

• product line: BioReagent
• Quality Segment: 100
• assay: ≥90.0% (HPLC), ≥90.0% (degree of coupling)
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• λ: in ethanol (with 0,1% trifluoroacetic acid)
• UV absorption: λ: 597-663 nm A_max
• suitability: suitable for fluorescence
• detection method: fluorometric
• storage temp.: −20°C
• SMILES string: [Cl](=O)(=O)(=O)[O-].[N+]1(=C2C(=CC3=C(c6c(cc7c(c6)C(=CC(N7CC)(C)C)C)OC3=C2)c4c(ccc(c4)C(=O)ON5C(=O)CCC5=O)C(=O)O)C(=CC1(C)C)C)CC
• InChI: 1S/C41H41N3O7.ClHO4/c1-9-42-31-18-33-29(16-26(31)22(3)20-40(42,5)6)37(30-17-27-23(4)21-41(7,8)43(10-2)32(27)19-34(30)50-33)28-15-24(11-12-25(28)38(47)48)39(49)51-44-35(45)13-14-36(44)46;2-1(3,4)5/h11-12,15-21H,9-10,13-14H2,1-8H3;(H,2,3,4,5)
• InChI key: SHJDVERDESTYRQ-UHFFFAOYSA-N

Beschreibung

General description

Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. The Atto dyes are a series of fluorescent dyes that provide all the crucial properties required for modern fluorescent technologies, such as fluorescence microscopy, flow-cytometry, fluorescence in situ hybridization (FISH), receptor binding assays or enzyme assays. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy. The NHS-esters are used in common conjugation protocols.

Application

Molare Absorption 120′000 1/M cm, Abs: 593 nm, Em: 620 nm, QY=0.93, T_fl 4.0 ns (unveröffentliche Daten)

Atto 590 NHS ester may be suitable for use in site-specific labeling of human embryonic kidney (HEK293T) cell lysates for western blotting, fluorescence, and widefield microscopy studies.[1]

Features and Benefits

• Absorption maxima cover a range from 590 to 620 nm.
• Structural properties support extraordinary photostability.
• High fluorescence quantum yield and high thermal stability.
• The fluorescence characteristics of the dye are quite insensitive to environmental changes (e.g., pH changes). This holds for both excitation and emission wavelengths and for the decay time of their fluorescence emission.
• Compatible with common labeling procedures for proteins and amino-functionalized oligonucleotides.

Other Notes

Untersuchung von multistep energy transfer in einem einzelnen photonischen Draht mit FRET[2]; Wird eingesetzt in Fluorescence Energy Transfer, FRET, Scanning Microscopy.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)