Yes. 20 grams of F2637 in 100ml of water should be the equivalent to the 20% Ficoll solution offered as catalog number F5415. It should however be noted that there is a density specification for product F5412. The density has to be within a range of 1.055 to 1.075 g/ml. Lot specific results will be reported on lot specific Certificates of Analysis.
Fortfahren mit
F5415
Ficoll® solution
Type 400, 20% in H2O
Synonym(e):
Ready-to-use density gradient media
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
25 mL | Warenkorb auf Verfügbarkeit prüfen | € 76,10 |
50 mL | Warenkorb auf Verfügbarkeit prüfen | € 129,00 |
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Technischer Dienst
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Unterstützung erhaltensterility
0.2 μm filtered
Quality Segment
type
Type 400
form
liquid
concentration
20% in H2O
technique(s)
titration: suitable
color
clear colorless
solubility
water: soluble
density
1.055-1.075 g/mL
application(s)
hematology
histology
storage temp.
2-8°C
General description
Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.
Application
Ficoll® has been used in
- Isolation of cord blood mononuclear cells
- Isolation of CD133 negative cells
- Isolation and expansion of circulating endothelial progenitor cells.
Other Notes
A nonionic synthetic polymer of sucrose.
Legal Information
Ficoll is a registered trademark of Cytiva
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Dieser Artikel | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) tissue culture: suitable | technique(s) - | technique(s) - |
| color clear colorless | color white to off-white | color white | color white |
| concentration 20% in H2O | concentration - | concentration - | concentration - |
| solubility water: soluble | solubility H2O: 100 mg/mL, clear to slightly hazy, colorless to faintly yellow | solubility water: soluble | solubility H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow |
| form liquid | form powder, spray-dried | form solid | form lyophilized powder |
| application(s) hematology | application(s) - | application(s) - | application(s) - |
Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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I have used Ficoll400 (F2637) from powder and consider to switch to your 20% solution. Is this product the same as dissolving e.g. 20 g of F2647 in water for a final volume of 100 mL?
1 answer-
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What are the differences between Ficoll, Ficoll Paque Plus, Ficoll Paque Premium, Histopaque and the different products with different densities?
1 answer-
1. Ficoll-Paque PREMIUM products - Sterile, ready-to-use density gradient media for the preparation of mononuclear cells - Based on Ficoll-Paque PLUS - Used for isolating high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood
2. Regulatory Status - Neither Ficoll-Paque Plus nor Ficoll-Paque Premium products are approved for In Vitro Diagnostic Use - For clinical studies, a GMP grade product (Ficoll-Paque Premium) is preferred over a product produced under ISO 9001 - Both products are sterile and low in endotoxins
3. Differences between Ficoll and Histopaque - Ficoll and Histopaque are essentially the same formulation - Ficoll is a trademark name for polysucrose 400, and Paque is a trademark name for sodium diatrizoate - Both can be used to separate various cell types based on different densities and species
4.Cell Separation Using Ficoll and Histopaque - Different densities are used for different cell types and sometimes the same cell types, but from different species - Ficoll and Histopaque can be used to separate a variety of cell types based on different densities and speciesHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can more Ficoll be added to Product F5415, Ficoll® solution 20%, to increase the density to 1.077 g/mL?
1 answer-
This is not recommended. Ficoll has a molecular weight in the range of 400,000. Even 20% solutions of such high molecular weight products have extremely low osmolality. Without the addition of other lower molecular weight chemical, solutions of 20%+ of Ficoll are considered extremely hypotonic. Cells exposed to hypotonic solutions would not maintain viability and are prone to bursting.
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Is Product F5415, Ficoll® solution 20% suitable for the separation of mononuclear cells without further dilution?
1 answer-
No. The 20% solution of Ficoll has a density of approximately 1.066 to 1.068 g/mL. Typically products such as Histopaque 1077-1 or Ficoll Paque have a density of approximately 1.076 to 1.078 g/mL. Such ready to use solutions are also osmotically balanced by the addition of a lower molecular weight chemical. Properly balanced solutions will typically have an osmolality in the range of approximately 280-300 mOsm/Kg. If a ready made product is required for the separation of mononuclear cells, products Histopaque 1077-1 or H8889 should be utilized.
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Is there a Product Information Sheet for Product F5415, Ficoll® solution 20%?
1 answer-
No. However there is a Product Information Sheet for product F4375. This product information sheet gives general information regarding Ficoll and possible uses. If mononuclear cells are to be separated, a better choice of products is Histopaque 1077-1, H8889 or one of the prefilled Accuspin tubes.
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Is Product F5415, Ficoll® solution 20%, sterile?
1 answer-
No claims are made for sterility for F5415. If a sterile product is required for cell culture use, product F8636 has been gamma irradiated and is considered a sterile product. Product F8636 is a powder and does not come as a sterile solution.
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What is the difference between Product F5415, Ficoll® solution 20%, and the polysucrose type 400 used in the Histopaque 1077-1?
1 answer-
Ficoll is a registered trademark. The trademark was formerly held by Pharmacia, but today Amersham Biosciences/GE Healthcare hold the trademark. Since Sigma Aldrich uses polysucrose type 400 in the preparation of the various Histopaque products, the name polysucrose 400 is utilized.
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