E9379

Stains-All

~95% purity, powder

• Synonym(e): 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-yliden)-2-methylpropenyl]-naphtho[1,2-d]thiazoliumbromid, 4,5,4′,5′-Dibenzo-3,3′-diethyl-9-methyl-thiacarbocyaninbromid
• Empirische Formel (Hill-System): C₃₀H₂₇BrN₂S₂
• CAS-Nummer: 7423-31-6
• Molekulargewicht: 559.58
• NACRES: NA.47
• PubChem Substance ID: 24894694
• UNSPSC Code: 12171500
• EC Number: 231-047-7
• MDL number: MFCD00078788
• Beilstein/REAXYS Number: 3865213

Eigenschaften

• Produktname: Stains-All, ~95%
• assay: ~95%
• Quality Segment: 200
• form: powder
• technique(s): microbe id | staining: suitable
• solubility: chloroform: 40 mg (PLUS 4.0 ml)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: [Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC
• InChI: 1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1
• InChI key: MPBRYMWMMKKRGC-UHFFFAOYSA-M

Beschreibung

Application

Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.

Biochem/physiol Actions

Stains-all is suitable for differential staining of nucleic acids and proteins as follows; RNA (bluish purple), DNA (blue) and proteins (red). As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Disclaimer

Bei längerer Lichteinwirkung wird der Nukleinsäure-Farbstoff-Komplex verblassen und nicht mehr sichtbar sein.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)