A8097

Acridinorange -hydrochlorid -Lösung

>95.0% purity (HPLC), solution

• Synonym(e): 3,6-Bis-(dimethylamino)-acridin -hydrochlorid
• Empirische Formel (Hill-System): C₁₇H₁₉N₃ · HCl
• CAS-Nummer: 65-61-2
• Molekulargewicht: 301.81
• NACRES: NA.47
• PubChem Substance ID: 24891317
• UNSPSC Code: 12171500
• MDL number: MFCD00012660
• Beilstein/REAXYS Number: 3575587

Eigenschaften

• Produktname: Acridinorange -hydrochlorid -Lösung, 10 mg/mL in H₂O, ≥95.0% (HPLC)
• Quality Level: 200
• assay: ≥95.0% (HPLC)
• form: solution
• concentration: 10 mg/mL in H₂O
• technique(s): microbe id | staining: suitable
• color: orange to red
• εmax: 47-54 at 486-492 nm in water
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cl[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
• InChI: 1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H
• InChI key: VSTHNGLPHBTRMB-UHFFFAOYSA-N

Beschreibung

Application

Metachromatischer Farbstoff zur Untersuchung von Mitochondrien in lebenden Zellen. Dieser Farbstoff wird benutzt um Mitochondrien per Durchflusszytometrie zu analysieren, Multiresistenzen zu charakterisieren und um Änderungen in der Mitochondrienmasse während der Apoptose in Ratten-Thymozyten zu messen.

Biochem/physiol Actions

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentration it intercalates into DNA and precipitates RNA. However, at high concentration it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It goes into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in the acidic organelle giving a bright red fluorescence.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Warning
• hcodes: H341
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)