This TE buffer solutions is not tested for pyrogen content, and it is not tested for sterility. Since it is not autoclaved or sterile filtered, it would be best to assume that it's not sterile as provided. This solution is recommended for Molecular Biology applications, which may not require the use of a pyrogen-free and/or sterile solution.
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93283
Tris-EDTA-Puffer -Lösung
BioUltra, Molecular Biology, pH 8.0
Synonym(e):
TE-Puffer, TE-Puffer -Lösung
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
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Über diesen Artikel
UNSPSC Code:
41105319
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25
MDL number:
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltengrade
Molecular Biology
Quality Level
product line
BioUltra
form
liquid
impurities
DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
pH
8.0±0.2 (25 °C)
cation traces
Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
λ
neat
UV absorption
λ: 260 nm Amax: 0.04, λ: 280 nm Amax: 0.01
application(s)
sample preparation
SMILES string
NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
InChI key
VLEIUWBSEKKKFX-UHFFFAOYSA-N
General description
Tris-EDTA ist ein biologischer Puffer in der Forschung. Es ist eine Kombination aus Tris-Base und EDTA, einem Metallchelator. Tris bietet Pufferkapazität und hält einen stabilen pH-Wert in biologischen Proben aufrecht, während EDTA dazu beiträgt, Interferenzen durch Metallionen durch Chelatbildung von divalenten Metallionen zu verhindern. Tris-EDTA wird häufig in molekularbiologischen Techniken wie DNA- und RNA-Probenvorbereitung, Elektrophorese und Enzymreaktionen verwendet. Es trägt zur Erhaltung der Stabilität und Integrität von Nukleinsäuren bei, indem es die Aktivität von Nukleasen und metallabhängigen Enzymen hemmt.
Application
TE-Puffer ist hilfreich in der allgemeinen Extraktion, Suspension, als Wasch- und Lagerpuffer sowie für die Präparation von DNA oder RNA. Er wird häufig für DNA-Sequenzierung, Restriktionsenzymverdauung, Ligation und Polymerase-Kettenreaktion (PCR) verwendet.
Features and Benefits
- BioUltra-Qualität – geeignet für viele molekularbiologische Anwendungen
- DNase-, RNase-, phosphatase- und proteasefrei
- Hohe Reinheit und frei von Spurenmetallen
Other Notes
Füllen Sie bitte dieses Formular aus, um weitere Informationen zu unserem Sortiment an Biochemikalien zu erhalten.
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Dieser Artikel | |||
|---|---|---|---|
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| product line BioUltra | product line BioUltra | product line BioUltra | product line BioReagent |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form liquid | form solution |
| application(s) sample preparation | application(s) - | application(s) - | application(s) - |
| pH 8.0±0.2 (25 °C) | pH 7.4±0.2 (25 °C) | pH 7.8±0.2 (25 °C) | pH - |
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Lagerklasse
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 93283-500ML | 04061833282533 |
| 93283-100ML | 04061838254573 |
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Is this product sterile and pyrogen free?
1 answer-
Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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Is this buffer composed by 10mM Tris and 1 mM EDTA?
1 answer-
This buffer is comprised of 10 mM Tris-HCl and 1 mM disodium EDTA, pH 8.0.
Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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What is the storage condition for TE buffer BioUltra
1 answer-
This product may be stored at room temperature.
Helpful?
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What is the concentration of Tris and EDTA in this buffer?
1 answer-
This buffer is 10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0.
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