This product is a Saline-Tris-EDTA (STE) buffer that is ready-to-use. It has a pH specification of 7.6 to 8.0, with a lot-specific pH provided on the certificate of analysis (CofA). This buffer is prepared using Tris base, which has a pH of about 11 in water; the pH is then lowered to the 7.6 to 8.0 range by using hydrochloric acid. Since the pKa of Tris is 8.1, one could say that the buffer is 10 mM in Tris, and just a little over 5 mM in HCl.
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85810
STE-Puffer -Lösung
BioUltra, Molecular Biology, pH 7.8
Synonym(e):
TRIS-NaCl-EDTA-Puffer -Lösung
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 100 mL | Warenkorb auf Verfügbarkeit prüfen | € 71,50 | |
| 500 mL | Warenkorb auf Verfügbarkeit prüfen | € 227,00 |
Über diesen Artikel
NACRES:
NA.25
UNSPSC Code:
41105319
Technischer Dienst
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Unterstützung erhaltengrade
Molecular Biology
Quality Level
product line
BioUltra
form
liquid
impurities
DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
pH
7.8±0.2 (25 °C)
cation traces
Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
λ
neat
UV absorption
λ: 260 nm Amax: 0.03, λ: 280 nm Amax: 0.01
Application
- Safer DNA extraction from plant tissues using sucrose buffer and glass fiber filter.: The study demonstrates a novel method for DNA extraction from plant tissues using STE buffer solution, ensuring higher safety and efficiency. This approach improves DNA yield and quality, essential for plant genetic research (Takakura et al., 2012).
- In vitro DNA fragmentation of mitochondrial DNA caused by single-stranded breakage related to macroplasmodial senescence of the true slime mold, Physarum polycephalum.: This study employs STE buffer solution to investigate mitochondrial DNA fragmentation in the slime mold Physarum polycephalum, providing valuable insights into cellular aging processes (Abe et al., 2000).
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Dieser Artikel | |||
|---|---|---|---|
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| product line BioUltra | product line BioUltra | product line BioUltra | product line - |
| form liquid | form liquid | form liquid | form liquid |
| pH 7.8±0.2 (25 °C) | pH 7.4±0.2 (25 °C) | pH 8.0±0.2 (25 °C) | pH 7.8-8.2 (1 × in water) |
| cation traces Al: ≤1 mg/kg, Bi: ≤1 mg/kg, Cd: ≤1 mg/kg, Cr: ≤1 mg/kg, Fe: ≤1 mg/kg, Li: ≤1 mg/kg, Mn: ≤1 mg/kg, Ni: ≤1 mg/kg, Sr: ≤1 mg/kg, Ba: ≤1 mg/kg, Co: ≤1 mg/kg, K: ≤20 mg/kg, Mo: ≤1 mg/kg, Zn: ≤1 mg/kg, Ca: ≤5 mg/kg, Mg: ≤1 mg/kg, Cu: ≤1 mg/kg, Pb: ≤1 mg/kg | cation traces Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg | cation traces Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg | cation traces - |
Lagerklasse
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 85810-500ML | 04061838169785 |
| 85810-100ML | 04061833032367 |
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Why do we prepare the buffer using Tris‑HCl and then adjust the pH to 8, instead of starting directly with Tris base since it is already closer to pH 8?
1 answer-
Helpful?
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Hello, is your item STE Buffer Molecular grade #85810 composed of these reagents: 0.1M NaCl, 1mM EDTA, 50mM Tris-HCl ? Is the pH stable overtime? Thank you for your reply.
1 answer-
This product is a Molecular Biology grade. The solution contains NaCl at 100 mM, Tris HCl at 10 mM, and Na2EDTA at 1 mM. The pH range is 7.6 - 8.0. The product has been filtered using a 0.22 um pore size filter membrane and is tested for RNase, DNase, Protease and Phosphatase activity.
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