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TR-1008

Sodium Butyrate Solution

This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells.

Synonym(e):

Cell Culture NaBu

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PackungsgrößeSKUVerfügbarkeitPreis
1 mL
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€ 53,00

Über diesen Artikel

UNSPSC Code:
12352207
NACRES:
NA.75
eCl@ss:
32160801

€ 53,00


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liquid

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sample type: mouse embryonic stem cell(s)

General description

This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells. Available as a 500mM stock solution (1mL). A complete protocol is included with shipment.

Application

Sodium butyrate can act to increase the efficiency of transfection and expression for both transient and stable transfections. It increases enhancer-dependant transcription of inserts driven by the SV40 early promoter. Vectors of this type include any of the pSV and pSV2 plasmids. Treatment of transfected cells with sodium butyrate can result in: a 2-3 fold increase in the number of cells able to express the insert; a 10-30 fold increase in the number of stable transfected colonies; and cells treated with sodium butyrate at the time of transfection can be re-induced by a second treatment weeks after the initial treatment and yield a 4-7 fold increase in transcription of the SV40 early promoter driven insert.



This treatment can be used on cells transfected by the Calcium Phosphate, DEAE Dextran and electroportation method.

Preparation Note

Store at 2-8°C for 2 years.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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303410P9327N-077
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sample type: mouse embryonic stem cell(s)

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form

liquid

form

powder

form

solution

form

liquid

manufacturer/tradename

Specialty Media

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Cerilliant®

Quality Level

200

Quality Level

200

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400

Quality Level

300


pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Lagerklasse

8B - Non-combustible corrosive hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Global Trade Item Number

SKUGTIN
TR-1008-G04053252504877

Questions

  1. I would like to know the stability of sodium butyrate once open, if stored at 4ºC. Do you have stability data also at -20ºC?

    1 answer
    1. Provided that the solution is handled properly and not contaminated, the product may be stored at 2-8°C for 2 years, either opened or unopened. This information is stated above, in the 'Description' section, under 'Preparation Note'. The stability of this solution when stored at -20 °C has not been determined.

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