This product is presented as a 40 mM solution in distilled water, at pH 4.1 ± 0.1, and is ready-to-use. The recommended volume is 1 ml for a 24-well plate and 0.5 ml for a 48-well plate.
TMS-008
Alizarin-Red Staining Solution
Alizarin red is a commonly used stain to identify calcium containing osteocytes in differentiated culture of both human and rodent mesenchymal stem cells (MSCs).
Synonym(e):
Alizarin Red S, Alizarin Red S Stain, Alizarin Red Stain
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 2 x 50 mL | Warenkorb auf Verfügbarkeit prüfen | € 124,00 |
Über diesen Artikel
NACRES:
NA.75
UNSPSC Code:
12352207
€ 124,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenQuality Level
concentration
40 mM in distilled water
technique(s)
cell based assay: suitable, cell culture | stem cell: suitable, cell differentiation: suitable
General description
Alizarin Red, an anthraquinone derivative, may be used to identify calcium in tissue sections and cultured cells in vitro. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining. Calcium forms an Alizarin Red S-calcium complex in a chelation process, and the end product is a bright red stain. Alizarin red is a commonly used stain to identify calcium containing osteocyte in differentiated culture of both human and rodent mesenchymal stem cells.
Application
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Osteobiology
Arthritis
Osteobiology
Arthritis
Physical form
Product is presented as a 40mM solution at pH 4.1 +/- 0.1.
Preparation Note
Store at 2-8ºC for up to 4 months from date of receipt. If Alizarin-red solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.
Analysis Note
Solution is red with no particulates present. Solution is suitable for endpoint cell culture assays.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Dieser Artikel | |||
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| technique(s) cell based assay: suitable, cell differentiation: suitable, cell culture | stem cell: suitable | technique(s) cell based assay: suitable, cell culture | stem cell: suitable, cell differentiation: suitable | technique(s) titration: suitable | technique(s) cell based assay: suitable, cell culture | stem cell: suitable, cell differentiation: suitable |
| concentration 40 mM in distilled water | concentration - | concentration 0.015 g/L in 0.1 M NaOH | concentration 0.1 % (w/v) in distilled water |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Analysenzertifikate (COA)
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Protokolle
Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.
Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.
Artikel
Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.
PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.
Verwandter Inhalt
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
We have collaborated with Plasticell Ltd. to develop OsteoMAX-XF™, a novel, serum-free, xeno-free, differentiation medium for the generation of osteoblasts from human MSCs. This medium may be particularly suitable for drug discovery and clinical development. By screening roughly 3,500 combinations of serum and xeno-free media, we discovered a remarkably efficient, fully defined, reproducible medium to promote the differentiation of hMSCs into osteoblasts, offering an excellent, cost effective method to produce large amounts of human bone cells for multiple applications.
"Apoptosis & Autophagy in Cancer: Cell Survival by Eluding Cell Death The ability of tumor cells to elude programmed cell death, also known as apoptosis, is a hallmark of most types of cancer. Similary, autophagy is a highly regulated, homeostatic degradative process where cells destroy their own components via the lysosomal machinery and recycle them for prolonged cell survival. Via extensive crosstalk with pro-apoptotic signaling pathways, autophagy can also contribute to cell death and greatly influence general cell health. Elucidating the correlation between autophagy and apoptotic cell death has become the focus of a great deal of research, particularly in tumor biology. On one hand, autophagy may induce cell death by degrading essential components; on the other hand, it may facilitate survival of cancer cells under unfavorable metabolic conditions."
Global Trade Item Number
| SKU | GTIN |
|---|---|
| TMS-008-C | 04053252914935 |
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Usually it is prepare a 2% solution for cell culture uses. This solution is used directly without any previous dilution?
1 answer-
Helpful?
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What difference between products TMS-008 Alizarin-Red Staining Solution and 2003999 Alizarin Red Solution?
1 answer-
Products TMS-008 and 2003999 are similar products. Product TMS-008 is presented as a 40mM solution at pH 4.1 +/- 0.1.
Product 2003999 is also a 40mM solution with pH range 4.1 +/- 0.1. It is also a kit component of Product ECM815.
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Dear Sir/Madam, Hope you are well. We already ordered ARS from your company. But we unfortunately lost the protocol inside the box. I was wondering if yo can email us the protocol. Best Regards, Ebrahim
1 answer-
Regarding your inquiry about TMS-008-C Alizarin-Red Staining Solution, there is currently no protocol available on sigmaaldrich.com for staining cells with TMS-008. The following information does appear on the emdmillipore.com website:
Product is presented as a 40mM solution at pH 4.1 +/- 0.1.As the molecular weight is 342.26, the percentage of Alizarin Red should be 1.4%. Most of the common histology textbooks generally recommend using a 2% concentration of Alizarin Red. But as in many histology procedures, the exact percentage is generally not critical for acceptable staining. It would appear the developers of the solution thought they preferred a concentration of 1.4%. This is typical of many histological staining procedures. Methods can and do vary from source to source.
As to a protocol, protocols for most histology textbooks should be acceptable for use. In general, tissue should not be fixed in fixative that contain calcium. Fixatives commonly used as 10% Neutral Buffered Formalin or alcoholic formalin. Once the slides have been prepared, hydrate sections in 50% alcohol. Then rinse briefly with distilled water. Incubate the slides in the Alizarin Red solution anywhere from 30 second to 5 minutes or more. Check for stain development every 30 seconds under a microscope until a red lake forms. The lake should not be too diffuse and on the heavy side. Then remove the solution by shaking off the excess and blot dry. Flood slides with acetone for about 20 seconds. Use a 50:50 mixture of acetone and xylene to differentiate. Clear in xylene. If possible, mount with a synthetic mounting media.
The solution does not have great stability. The website further states: Store at 2-8ºC for up to 4 months from date of receipt. If Alizarin-red solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.
This product is included in some of our kits, such as ECM815 or ECM810. Here are the user guides for the kit ECM815 and ECM810, which contains information about the Alizarin red solution protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/190/283/ecm810man.pdf
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/119/ecm815.pdfYou can also find application information for Alizarin Red at the following links:
https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/stem-cell-culture/mesenchymal-stem-cell-culture-protocolsHelpful?
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