SCC197

NP69 Human Nasopharyngeal Epithelial Cell Line

The NP69 cell line is useful for studies of the mechanisms of viral-associated tumorigenesis

• Synonym(e): NP69SV40T, NP-69
• NACRES: NA.45
• UNSPSC Code: 41106514

Eigenschaften

• technique(s): cell culture | mammalian: suitable
• input: epithelial cells

Beschreibung

General description

The NP69 immortalized human nasopharyngeal epithelial cell line retains many of the characteristics of normal nasopharyngeal cells, including keratin profiles and response to TGF-beta, while possessing many of the genetic signatures observed in NPC (2). NP69 cells are non-tumorigenic and demonstrate anchorage-dependent growth (2). The NP69 cell line is particularly useful for studies of the mechanisms of viral-associated tumorigenesis. Source: NP69 cells were derived from passage 1 cultures of biopsy-isolated primary nasopharyngeal epithelial cells transfected with the PX8 plasmid containing the simian virus 40 large T antigen (1).

Nasopharyngeal carcinoma (NPC), the most common cancer arising in the nasopharyngeal region, arises from epithelial cells and is especially prevalent in areas of southeast Asia and as a childhood cancer in some African populations (1). NPC ontology and tumorigenesis is closely associated with viral factors, especially Epstein-Barr virus (EBV) and human papillomavirus (HPV) (1).

References: 1. Lancet 2016; 387(10022): 1012-1024. 2. Biochim Biophys Acta 2002; 1590(1-3): 150-158.

Application

The NP69 cell line is useful for studies of the mechanisms of viral-associated tumorigenesis.
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].

Packaging

≥1X10⁶ cells/vial

Preparation Note

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Analysis Note

• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Clickhere for Enduser Declaration (EUD) Form.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable