NS03L

Normal Mouse Serum

This Normal Mouse Serum is validated for use in ELISA, Flow Cytometry, Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation for the detection of Mouse Serum.

• Synonym(e): Mouse Serum
• NACRES: NA.43
• UNSPSC Code: 12352202
• Biological source: mouse
• Form: lyophilized
• Shipped in: ambient

Eigenschaften

• biological source: mouse
• form: lyophilized
• contains: ≤0.1% sodium azide as preservative
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• dilution: (ELISA ; Flow Cytometry ; Immunoblotting ; Immunofluorescence ; Immunohistochemistry ; Immunoprecipitation )
• isotype: IgG
• shipped in: ambient
• storage temp.: 2-8°C

Beschreibung

General description

Normal carrier sera from healthy, non-immunized mice.

Application

ELISA (see comments)

Flow Cytometry (see comments)

Immunoblotting (see comments)

Immunofluorescence (see comments)

Immunohistochemistry (see comments)

Immunoprecipitation (see comments)

Physical form

Lyophilized serum. Lyophilized from 250 mM sodium chloride, 10 mM sodium phosphate, pH 7.6.

Preparation Note

Reconstitute the lypohilized serum in 1 ml of sterile distilled water with occasional gentle mixing for at least 2 h at 4°C. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

Other Notes

Useful as a blocking agent in immunohistochemical procedures or as a negative control for specific mouse antibodies. For use as a negative control in any application, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction. This antibody has also been reported to work for ELISA, flow cytometry, immunoblotting, immunofluorescence, immunohistochemistry, and immunoprecipitation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Lagerklasse: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable