NE1019

Anti-Myelin Basic Protein Mouse mAb (SMI-99)

liquid, clone SMI-99, Calbiochem^®

• Synonym(e): Myelin basic protein antibody
• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: SMI-99, monoclonal
• Species reactivity: guinea pig (weakly), mammals
• Application: —
• Citations: 22

Eigenschaften

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: SMI-99, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: guinea pig (weakly), mammals
• should not react with: chicken, porcine
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (ELISA (1:1000); Immunoblotting (1:1000); Immunocytochemistry (1:1000); Frozen Sections (1:1000); Paraffin Sections (1:1000))
• isotype: IgG2b
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MBP(4155)

Beschreibung

General description

Recognizes the ~21 kDa myelin basic protein in rat brain tissue. Does not recognize the 14 kDa form. Recognizes most mammalian species.

This Anti-Myelin Basic Protein Mouse mAb (SMI-99) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Frozen Sections, Paraffin Sections for the detection of Myelin Basic Protein.

Purified mouse monoclonal antibody. Recognizes the ~21 kDa myelin basic protein (MBP).

Immunogen

Epitope: Within amino acids 131-136

human myelin basic protein

Application

ELISA (1:1000)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000)
Frozen Sections (1:1000)
Paraffin Sections (1:1000)

Other Notes

Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods72, 197.
Shin, R.W., et al. 1991. Lab. Invest.64, 693.

Recognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable