MABS61
Anti-PARG Antibody, clone D8B10
clone D8B10, from mouse
Synonym(e):
poly (ADP-ribose) glycohydrolase, poly(ADP-ribose) glycohydrolase 60 kDa isoform
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About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified immunoglobulin
Antikörper-Produkttyp
primary antibodies
Klon
D8B10, monoclonal
Speziesreaktivität
human
Methode(n)
immunoprecipitation (IP): suitable
western blot: suitable
Isotyp
IgG2aκ
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
wet ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... PARG(8505)
Allgemeine Beschreibung
Poly(ADP-ribose) glycohydrolase (PARG) is an enzyme possessing both endo- and exoglycosidase activity against poly (ADP-ribose) (PARP), rapidly degrading PARP to release large quantities of free ADP-ribose. PARG is involved in several cellular processes including; apoptosis, DNA repair, cell cycle progression, cell survival and cellular differentiation. During apoptosis, PARG is cleaved by caspase-3 suggesting that PARG activity is regulated during this process. Although encoded by one gene, PARG is present in different cellular localizations as different isoforms: Isoform 1 (111 kDa) is present in the nucleus, Isoform 2 (102 kDA) is in the cytoplasm and Isoform 3 (99 kDa) is mitochondrial. Studies have indicated a critical role for PARG isoform 1 in the quality of sperm chromatin, and subsequent embryonic survival.
Immunogen
Epitope: Unknown
Histidine-tagged recombinant protein corresponding to human PARG.
Anwendung
Research Category
Zelluläre Signaltransduktion
Epigenetik & nukleäre Funktionen
Zelluläre Signaltransduktion
Epigenetik & nukleäre Funktionen
Research Sub Category
Hormone & Rezeptoren
Chromatin-Biologie (ChIP)
Hormone & Rezeptoren
Chromatin-Biologie (ChIP)
Anti-PARG Antibody, clone D8B10 is an antibody against PARG for use in WB & IP.
Qualität
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PARG on 10 µg of Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PARG on 10 µg of Jurkat cell lysate.
Zielbeschreibung
~ 102 kDa observed. Other isoforms may be observed in some lysates at 111 and 99 kDa
Physikalische Form
Protein G
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Lagerung und Haltbarkeit
Stable for 1 year at 2-8°C from date of receipt.
Hinweis zur Analyse
Control
Jurkat cell lysate
Jurkat cell lysate
Sonstige Hinweise
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Jean-Christophe Amé et al.
Methods in molecular biology (Clifton, N.J.), 1608, 395-413 (2017-07-12)
The purification of Poly(ADP-ribose) glycohydrolase (PARG) from overexpressing bacteria Escherichia coli is described here to a fast and reproducible one chromatographic step protocol. After cell lysis, GST-PARG-fusion proteins from the crude extract are affinity purified by a Glutathione 4B Sepharose
Yajie Zhang et al.
Nature methods, 10(10), 981-984 (2013-08-21)
Poly(ADP-ribosyl)ation is catalyzed by a family of enzymes known as PARPs. We describe a method to characterize the human aspartic acid- and glutamic acid-ADP-ribosylated proteome. We identified 1,048 ADP-ribosylation sites on 340 proteins involved in a wide array of nuclear
Kaede Tsuda et al.
Cancer reports (Hoboken, N.J.), 6(2), e1709-e1709 (2022-09-03)
Poly(ADP-ribose) glycohydrolase (PARG) is a key enzyme in poly(ADP-ribose) (PAR) metabolism and a potential anticancer target. Many drug candidates have been developed to inhibit its enzymatic activity. Additionally, PDD00017273 is an effective and selective inhibitor of PARG at the first
Xiaoxing Feng et al.
Molecular cancer, 11, 48-48 (2012-07-31)
Cell death induced by poly(ADP-ribose) (PAR) and mediated by apoptosis-inducing factor (AIF) is well-characterized in models of ischemic tissue injury, but their roles in cancer cell death after chemotherapy are less understood. Here we investigated the roles of PAR and
Francesco Morra et al.
Journal of experimental & clinical cancer research : CR, 41(1), 245-245 (2022-08-14)
Treatment with PARP inhibitors (PARPi) is primarily effective against high-grade serous ovarian cancers (HGSOC) with BRCA1/2 mutations or other deficiencies in homologous recombination (HR) repair mechanisms. However, resistance to PARPi frequently develops, mostly as a result of BRCA1/2 reversion mutations.
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