MABF918

Anti-EAT-2 Antibody, clone 8F12

clone 8F12, from rat

• Synonym(e): SH2 domain-containing protein 1B, EWS/FLI1-activated transcript 2
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 8F12, monoclonal
• Speziesreaktivität: mouse, human
• Methode(n): immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG2aκ
• UniProt-Hinterlegungsnummer: O35324
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SH2D1B(117157)

Beschreibung

Allgemeine Beschreibung

SH2 domain-containing protein 1B (UniProt: O35324; also known as EWS/FLI1-activated transcript 2, EAT-2) is encoded by the Sh2d1b (also known as Eat2, Eat2a, Sh2d1b1) gene in murine species. EAT-2 is a Src homology 2 (SH2) - domain-containing (aa 5-101) intracellular adaptor protein that is related to signaling lymphocytic activation molecule (SLAM) associated protein (SAP). EAT-2 and SAP are expressed in natural killer (NK) cells, and their combined expression appears to be essential for NK cells to kill abnormal hematopoietic cells. NK cells lacking either SAP or EAT-2 display partial defects in NK cell-mediated cytotoxicity toward abnormal hematopoietic cells and more severe defects are observed when NK cells lacking both adaptors. It plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through CD244/2B4. It is proposed to act as an inhibitor of CD244-mediated NK cell function including cytotoxicity and interferon-gamma production. It is reported to repress natural cytotoxicity and interferon secretion by a mechanism involving tyrosine phosphorylation of its C-terminus. It binds to the phosphorylated receptors CD84, SLAMF1, LY9 and CD244 and does not bind to non-phosphorylated SLAMF1. Mice lacking EAT-2 are reported to have enhanced NK cell activation responses. EAT-2 is expressed at all stages of maturation in NK cells and in macrophages and dendritic cells. EAT-2 is also expressed in spleen, thymus, lung, kidney, heart, colon and small bowel. (Ref.: Luis-Alberto Pérez-Quintero, L-A., et al. (2014). J. Exp. Med. 211(4); 727-742).

Spezifität

Clone 8F12 detects EWS/FLI1-activated transcript 2 (EAT-2) in human and murine cells.

Immunogen

GST-tagged full length recombinant murine SH2 domain-containing protein 1B (EAT-2).

Anwendung

Research Category
Zelluläre Signaltransduktion

Anti-EAT-2, clone 8F12, Cat. No. MABF918, is a rat monoclonal antibody that detects SH2 domain-containing protein 1B (EAT-2) and has been tested for use in Western Blotting and Immunoprecipitation.

Immunoprecipitation Analysis: A representative lot immunoprecipitated EAT-2 (Perez-Quintero, L.A., et. al. (2014). J Exp Med. 211(4):727-42).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected EAT-2 in YT-S transfectants expressing mouse EAT-2 and YT-S wild type cell lysates (Courtesy of Ming-Chao Zhong, Research Associate for Dr. Andre Veillette, Laboratory of Molecular Oncology, Clinical Research Institute of Montreal, Montreal, Quebec, Canada.).

Western Blotting Analysis: A representative lot detected EAT-2 in Western Blotting applications (Cruz-Munoz, M.E., et. al. (2009). Nat Immunol. 10(3):297-305; Guo, H., et. al. (2009). Mol Cell Biol. 35(1):41-51; Perez-Quintero, L.A., et. al. (2014). J Exp Med. 211(4):727-42.

Qualität

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be rat IgG2a.

Zielbeschreibung

15.26 kDa calculated.

Physikalische Form

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1