MAB5384A4

Anti-NG2 Antibody, clone 132.39, Alexa Fluor^™488 Conjugate

clone 132.39, from mouse, ALEXA FLUOR^™ 488

• Synonym(e): Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: ALEXA FLUOR^™ 488
• Clone: 132.39, monoclonal
• Application: ICC
• Citations: 3

Eigenschaften

• biological source: mouse
• Quality Level: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 132.39, monoclonal
• species reactivity: rat
• species reactivity (predicted by homology): mouse, human
• technique(s): immunocytochemistry: suitable
• isotype: IgG1
• NCBI accession no.: NP_112284
• UniProt accession no.: Q00657
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CSPG4(1464) ; mouse ... Cspg4(121021) ; rat ... Cspg4(81651)

Beschreibung

General description

NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.

The unconjugated parent antibody (Cat. No. MAB5384) has an observed molecular weight at ~280 kDa

Immunogen

Cell line expressing a truncated form of NG2

Application

Anti-NG2 Antibody, clone 132.39, Alexa Fluor^™488 Conjugate detects level of NG2 & has been published & validated for use in ICC.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Biochem/physiol Actions

This antibody recognizes the native, recombinant, and protein from cells which express NG2.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Preparation Note

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Rat oligodendrocyte precursor cells

Evaluated by Immunocytochemistry in rat oligodendrocyte precursor cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NG2 in rat oligodendrocyte precursor cells. Performed without Triton.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable