MAB4385

Anti-TRA-1-85 Antibody, blood group Antigen Ok(a), clone TRA-1-85

clone TRA-1-85, Chemicon^®, from mouse

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: TRA-1-85, monoclonal
• Species reactivity: human
• Application: FACS, IP, WB
• Citations: 5

Eigenschaften

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: TRA-1-85, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• input: sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• isotype: IgG1
• shipped in: wet ice
• target post-translational modification: unmodified

Beschreibung

General description

35-69 kDa

The high-frequency blood group antigen Ok(a) is carried on a red cell membrane glycoprotein (gp) of 35-69 kDa that is widely distributed on malignant cells of different origins. Immunostaining of hemopoietic cells and a range of normal human tissues demonstrated a wide distribution of the Ok(a) gp that appears to be nonlineage-restricted, although certain tissues show differentiation-related expression. This clone can be used to monitor contamination of human Embryonic Stem cell cultures with murine feeder cells.

Immunogen

2102Ep human Embryonal Carcinoma Cells

Epitope: blood group antigen Ok(a)

Application

Anti-TRA-1-85 Antibody, blood group antigen Ok(a), clone TRA-1-85 detects level of TRA-1-85 & has been published & validated for use in FC, IP & WB.

FACS Analysis: a starting range of 5-20 μg/ml is suggested.

Immunoprecipitation

Immunoblotting

Optimal working dilutions must be determined by the end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Biochem/physiol Actions

This antibody exhibits pan-human reactivity, recognizing the cell surface blood group antigen Ok (a). Reactivity to murine cells has not been observed.

SPECIES REACTIVITIES:

Refer to comments regarding specificity

Physical form

Format: Purified

Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25 NaCl ph 7.6 with 0.1% Sodium Azide

Preparation Note

Maintain refrigerated at 2-8C in undiluted aliquots for up to 12 months

Analysis Note

Control
Hemopoietic cells and a wide range of normal human tissues

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Lagerklasse: 12 - Non Combustible Liquids