MAB3319

Anti-MMP-14 Antibody, catalytic domain, clone 114-6G6

clone 114-6G6, Chemicon^®, from mouse

• Synonym(e): MT1-MMP
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 114-6G6, monoclonal
• Application: ELISA, IHC, WB
• Citations: 9

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 114-6G6, monoclonal
• species reactivity: human
• should not react with: pig, rat, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NM_004995.2
• UniProt accession no.: P50281
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... MMP14(4323)

Beschreibung

Immunogen

Epitope: catalytic domain

Application

Immunoblotting: 8-20 μg/mL. 66+60kDa (Ueno et al., 1997)

Immunohistochemistry on paraffin-embedded tissue sections, use at 20 μg/mL concentration (see Ueno et al., 1997; Nakamura et al., 1999). Requires periodate-lysine-paraformaldehyde fixative (Namura et al., 1999) for 18-24 hrs. at 4°C.

EIA Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

This Anti-MMP-14 Antibody, catalytic domain, clone 114-6G6 is validated for use in ELISA, WB, IH(P) for the detection of MMP-14.

Biochem/physiol Actions

The antibody specifically reacts with human MT1-MMP. This is a purified mouse monoclonal antibody to an oligopeptide of REVPYAYIREGHEK (residue 160-173) on human membrane-type 1 matrix metalloproteinase (human MT1-MMP) and does not react with mouse or rat. However, this sequence is identical in mouse and rat MT1-MMP. For unknown reasons this antibody does not react on westerns to CC1043.

Physical form

Format: Purified

Liquid in 0.1 M sodium phosphate buffer, pH 7.0 containing 2% protease free bovine serum albumin.

Preparation Note

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Manufactured by Daiichi Fine Chemical Co., Ltd

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable