MAB3317

Anti-MMP-14 Antibody, hemopexin domain, clone 113-5B7

clone 113-5B7, Chemicon^®, from mouse

• Synonym(e): MT1-MMP
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 113-5B7, monoclonal
• Application: ELISA, IHC, WB
• Citations: 27

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 113-5B7, monoclonal
• species reactivity: rat, human, bovine, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG3κ
• NCBI accession no.: NM_004995.2
• UniProt accession no.: P50281
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... MMP14(4323)

Beschreibung

General description

~59-69 kDa

Immunogen

CDGNFDTVAMLRGEM in the hemopexin-like domain of human MT1-MMP

Epitope: a.a. 319-333

Application

Anti-MMP-14 Antibody, hemopexin domain, clone 113-5B7 is an antibody against MMP-14 for use in ELISA, IH(P) & WB.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Western Blot: 10 μg/mL, membrane preparations; MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours as positive control. 60-65 kDa proteins are identified.


When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59 kDa.


Immunohistochemistry: Frozen tissue sections; traditional formalin fixation is problematic. Paraffin-embedded tissue sections with PLP fixation required.


Immunoprecipitation


EIA


Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Reacts with human MMP-14, also known as MT1-MMP, with a very slight cross-reactivity to hMMP-3. For unknown reasons this antibody does not react on westerns to CC1043.

Physical form

Format: Purified

Protein A purified

Purified immunoglobulin. Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine Serum albumin.

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Breast carcinoma tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Manufactured by Daiichi Fine Chemical Co., Ltd

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable