MAB1254B

Anti-Chymase Antibody, clone B7, biotin conjugated

clone B7, Chemicon^®, from mouse

• Synonym(e): Mast Cell Protease I
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: biotin conjugate
• Clone: B7, monoclonal
• Application: DB, IHC
• Citations: 22

Eigenschaften

• biological source: mouse
• Quality Level: 100
• conjugate: biotin conjugate
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: B7, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): dot blot: suitable, immunohistochemistry: suitable
• isotype: IgG1κ
• suitability: not suitable for Western blot
• NCBI accession no.: NM_001836.2
• UniProt accession no.: P23946
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CMA1(1215)

Beschreibung

General description

Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.

Immunogen

Chymase purified from human skin.

Application

Immunohistochemistry at 1:2,000:1:4,000.

For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

Not recommended for use on Western blots.

Optimal working dilutions must be determined by end user.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

This Anti-Chymase Antibody, clone B7, biotin conjugated is validated for use in DB, IH for the detection of Chymase.

Biochem/physiol Actions

Reacts with human mast cell chymase.

Physical form

Culture supernatant, purified by ammonium sulfate precipitation and DEAE-Sephacyl chromatography, labeled with biotin. In 0.01M PBS, 15 mg/mL BSA, 0.1% sodium azide, pH 7.1.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Analysis Note

Control
POSITIVE CONTROL: lung, heart, skin, placenta.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable