IM42

Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6)

liquid, clone 114-6G6, Calbiochem^®

• Synonym(e): Anti-MMP-14
• UNSPSC Code: 12352203
• Clone: 114-6G6, monoclonal
• Species reactivity: human
• Application: —
• Citations: -

Eigenschaften

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 114-6G6, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles

Beschreibung

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~66 kDa latent and the ~60 kDa active forms of MT1-MMP protein.

Recognizes the ~60-66 kDa MTI-MMP protein in HT-1080 cells and ConA-treated MD-MB-231 cells.

This Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6) is validated for use in Frozen Sections, Paraffin Sections, Immunoblotting, Immunocytochemistry, IP for the detection of MT1-MMP (Ab-3).

Immunogen

a synthetic peptide (REVPYAYIREGHEK) corresponding to amino acids 160-173 in the catalytic domain of human MT1-MMP

Application

Frozen Sections (not recommended)
Paraffin Sections (10 g/ml, Pronase<TMSYMBOL></TMSYMBOL> or heat pre-treatment required)
Immunoblotting (10 g/ml)
Immunocytochemistry (see application reference)
Immunoprecipitation (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
trpE (Ab-1) or untreated MD-MB-231 cells

Positive Control
HT-1080 cells, ConA-treated MD-MB-231 cells, or breast carcinoma tissue

Other Notes

Does not cross-react with MT2-MMP or MT3-MMP. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. For best results with paraffin sections, fix with perioddate-lysine-4% paraformaldehyde. Antibody should be titrated for optimal results in individual systems.

Mattei, M.G., et al. 1997. Genomics40, 168.
Okada, A., et al. 1997. J. Cell. Biol.147, 67.
Okumura, Y., et al. 1997. FEBS Lett.402, 181.
Ueno, H., et al. 1997. Cancer Res.57, 2055.
Strongin, A.Y., et al. 1995. J. Biol. Chem.270, 5331.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Sato, H., et al. 1994. Nature370, 61.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.
Fridman, R., et al. 1992. J. Biol. Chem.267, 15389.
Woessner, J.F. 1991. FASEB J.5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M.M. Gottesman. Vol. 1(2):99-106.
Goldberg, G.I., et al. 1989. PNAS86, 8207.

Legal Information

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1