Reconstitute in sterile PBS with 0.1% endotoxin-free human serum albumin.
GF446
Thermostable bFGF,Human
HumanKine®, recombinant, Xeno-free, >95% (SDS-PAGE), suitable for cell culture
Synonym(e):
FGF2, prostatropin, Prostatropin, HBGF-2, BFGF
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 10 μg | Warenkorb auf Verfügbarkeit prüfen | € 173,00 | |
| 50 μg | Warenkorb auf Verfügbarkeit prüfen | € 708,00 |
Über diesen Artikel
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Assay:
>95% (SDS-PAGE)
Impurities:
<0.5 μg/mg protein endotoxin (<5 EU/μg)
€ 173,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenProduktname
HumanKine® Thermostable bFGF,
Human Recombinant, Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.
Quality Level
assay
>95% (SDS-PAGE)
manufacturer/tradename
HumanKine®
impurities
<0.5 μg/mg protein endotoxin (<5 EU/μg)
UniProt accession no.
General description
Product Source: HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.
The HumanKine Thermostable bFGF is engineered with enchanced thermostability compared to the wild-type bFGF protein without modification of biological function.
Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 154 amino acid residues. bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions.
Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 154 amino acid residues. bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions.
Application
Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.
HumanKine products are Xeno-Free
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Physical form
Lyophilized from a 0.2 μm filtered solution of 1X PBS.
Preparation Note
Store at -20°C for up to 4 months from date of receipt. Further dilutions should be made into appropriate buffer or medium to which protein has been added.
Analysis Note
Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of the 3T3 cell line. EC50 is typically ≤0.5 ng/mL.
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Dieser Artikel | |||
|---|---|---|---|
| assay >95% (SDS-PAGE) | assay ≥95% (SDS-PAGE and HPLC) | assay >95% (SDS-PAGE) | assay ≥95% (HPLC), ≥95% (SDS-PAGE) |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level - |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| impurities <0.5 μg/mg protein endotoxin (<5 EU/μg) | impurities 1 EU/μg | impurities <0.1 EU/μg Endotoxin (of bFGF) | impurities endotoxin, tested |
| manufacturer/tradename HumanKine® | manufacturer/tradename - | manufacturer/tradename Chemicon® | manufacturer/tradename - |
Lagerklasse
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| GF446-10UG | 04053252975943 |
| GF446-50UG | 04053252975950 |
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What can be used to dilute this bFGF in order to make aliquots?
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