The concentration refers to the 10 µL loaded and is the concentration to be entered for the standard concentration in the analysis software. Refer to protocol page 7, Rat/Mouse Growth Hormone Standard Preparation, for the standard curve values.
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Über diesen Artikel
sample type cell culture supernatant
sample type plasma (K2 EDTA)
(10 μl sample size), standard curve range: 0.07-50 ng/mL
Produktname
Ratten-/Maus-Wachstumshormon ELISA, This Rat/Mouse Growth Hormone ELISA is used to measure & quantify Growth Hormone levels in Metabolism & Endocrine & Cell Signaling research.
Quality Segment
species reactivity
rat, mouse
packaging
kit of 1 × 96 wells
parameter
10 μL sample volume (4hr assay)
assay range
accuracy: 96-101%, linearity: 97-118%, sensitivity: 0.07 ng/mL
(10 μl sample size), standard curve range: 0.07-50 ng/mL
technique(s)
ELISA: suitable
input
sample type serum
sample type cell culture supernatant
sample type plasma (K2 EDTA)
application(s)
research use
detection method
colorimetric (450nm/590nm)
shipped in
wet ice
storage temp.
2-8°C
General description
Application
Other Notes
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| species reactivity rat, mouse | species reactivity human | species reactivity mouse, rat | species reactivity human |
| assay range accuracy: 96-101%, sensitivity: 0.07 ng/mL | assay range accuracy: 86.7%, linearity: 99.9%, sensitivity: 8.2 pg/mL, standard curve range: 8.2-2000 pg/mL | assay range accuracy: 83-102%, linearity: 79-133%, sensitivity: 0.2 ng/mL, standard curve range: 0.2-10 ng/mL | assay range - |
| input sample type serum | input sample type cell culture supernatant | input sample type serum | input sample type plasma (K2 EDTA) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| detection method colorimetric (450nm/590nm) | detection method colorimetric (450nm/590nm) | detection method colorimetric (450nm/590nm) | detection method colorimetric (450nm/590nm) |
| technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable |
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Lagerklasse
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
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With regard to what is loaded into each well (10 ul sample or standard plus 90 ul assay buffer) does the standard concentration (e.g 50 ng/ml) refer to the concentration of the 10 ul, or of the final 100 ul in the well? Thank you.
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