Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and lambda light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
Spezifität
Reacts with human kappa light chains. Absorbed for human myeloma proteins with λ light chains.
The antibody reacts with human kappa light chains as demonstrated by ELISA and flow cytometry.
Immunogen
Prepared from pooled antisera from goats hyperimmunized with human myeloma proteins with kappa light chains.
Anwendung
Research Category Sekundär- & Kontrollantikörper
Research Sub Category Fragmentspezifische Sekundärantikörper
Detect Human Ig κ chain using this Goat anti-Human Ig κ chain Antibody, Alkaline Phosphatase conjugate validated for use in ELISA.
ELISA: 1:2,000-1:4,000.
Optimal working dilutions must be determined by the end user.
Physikalische Form
Affinity Chromatography
Purified by affinity chromatography on pooled human Igs with kappa light chains covalently linked to agarose. Liquid in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative.
Lagerung und Haltbarkeit
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
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