AP505A

Goat Anti-Human Ig κ chain Antibody, Alkaline Phosphatase conjugate

Chemicon^®, from goat

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: alkaline phosphatase conjugate
• Clone: polyclonal
• Application: ELISA
• Citations: -

Eigenschaften

• biological source: goat
• Quality Segment: 100
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity purified immunoglobulin
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable
• shipped in: wet ice
• target post-translational modification: unmodified

Beschreibung

General description

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and lambda light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Immunogen

Prepared from pooled antisera from goats hyperimmunized with human myeloma proteins with kappa light chains.

Application

Detect Human Ig κ chain using this Goat anti-Human Ig κ chain Antibody, Alkaline Phosphatase conjugate validated for use in ELISA.

ELISA: 1:2,000-1:4,000.

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

Biochem/physiol Actions

Reacts with human kappa light chains. Absorbed for human myeloma proteins with λ light chains.

The antibody reacts with human kappa light chains as demonstrated by ELISA and flow cytometry.

Physical form

Affinity Chromatography

Purified by affinity chromatography on pooled human Igs with kappa light chains covalently linked to agarose. Liquid in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN₃ as preservative.

Preparation Note

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1