ABE499

Anti-JMJD2D Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Lysine-specific demethylase 4D, JmjC domain-containing histone demethylation protein 3D, Jumonji domain-containing protein 2D
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): chimpanzee (based on 100% sequence homology)
• Methode(n): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_060509
• UniProt-Hinterlegungsnummer: Q6B0I6
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... KDM4D(55693)

Beschreibung

Allgemeine Beschreibung

Jumonji domain-containing protein 2D (JMJD2D) is also called Lysine-specific demethylase 4D and JmjC domain-containing histone demethylation protein 3D. JMJD2D is a histone demethylase that specifically demethylates both di- and trimethylated H3 ′Lys-9′, but not monomethylated histone H3 ′Lys-9′ residues. JMJD2D also does not demethylate histone H3 ′Lys-4′, H3 ′Lys-27′, H3 ′Lys-36′ or H4 ′Lys-20′.

Spezifität

Other homologies: Bovine, Porcine, Canine, and Rhesus Macaque (93% sequence homology), Mouse (87% sequence homology), Rat (80% sequence homology).

Immunogen

KLH-conjugated linear peptide corresponding to human JMJD2D.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Chromatin-Biologie (ChIP)

Detect Jumonji domain-containing protein (JMJD) using this rabbit polyclonal antibody, Anti-JMJD2D Antibody validated for use in western blotting, IHC & IP.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected JMJD2D in human testis and liver tissues.

Immunoprecipitation Analyis: 20 µg from a representative lot detected JMJD2D in 500 µg of HeLa cell lysate.

Qualität

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected JMJD2D in 10 µg of HeLa cell lysate

Zielbeschreibung

~58 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
HeLa cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable