AB5539 is the anti Anti-Neurofilament H (200 kDa) Antibody. I does stain the cytoplasmic filaments in neuronal cell bodies, and especially axons. In my opinion, most likely the weaker and stonger staining pattern is due to the NF antibody reacting to phosphorylated epitope in the axon. You may wish to review genral literature on this line, e.g. literature PMID: 3133402, PMID: 6416876,
PMID: 2998843
AB5539
Anti-Neurofilament H (200 kDa) Antibody
CHEMICON®, chicken polyclonal
Synonym(e):
NF-H
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Ansicht ändern
| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 50 μL | Warenkorb auf Verfügbarkeit prüfen | € 677,00 |
Über diesen Artikel
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
rat, pig, bovine, feline, human, mouse
Application:
ICC, IHC (p), WB
Citations:
192
€ 677,00
Warenkorb auf Verfügbarkeit prüfen
Für Ihr Target ist ein rekombinanter, konservierungsmittelfreier Antikörper verfügbar. Probieren Sie ZRB1347
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenProduktname
Anti-Neurofilament H Antikörper, Chemicon®, from chicken
biological source
chicken
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, pig, bovine, feline, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
bovine ... Nefh(528842)
cat ... Nefh(101087272)
human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)
General description
200 kDa
Immunogen
Aufgereinigter NF-H des Rindes.
Application
Anti-Neurofilament-H-Antikörper ist ein Antikörper gegen das Neurofilament und wird in IC, IH, IH(P) und WB verwendet.
Western Blot: 1:1.000–1:10.000 mittels ECL. Erkennt eine -220-kDa-Bande.
Immunhistochemie: 1:1.000 – 1:10.000 bei gefrorenen oder in Paraffin eingebetteten Gewebeschnitten. Empfohlenes Fixierungsmittel ist 4 % Paraformaldehyd.
Immunzytochemie: 1:1.000 – 1:10.000 auf Zellen in Gewebekultur, eine Minute lang in 3,7%igem Formalin und eine Minute in -20 °C kaltem Methanol fixiert.
Optimale Arbeitsverdünnungen müssen vom Endbenutzer bestimmt werden.
Immunhistochemie: 1:1.000 – 1:10.000 bei gefrorenen oder in Paraffin eingebetteten Gewebeschnitten. Empfohlenes Fixierungsmittel ist 4 % Paraformaldehyd.
Immunzytochemie: 1:1.000 – 1:10.000 auf Zellen in Gewebekultur, eine Minute lang in 3,7%igem Formalin und eine Minute in -20 °C kaltem Methanol fixiert.
Optimale Arbeitsverdünnungen müssen vom Endbenutzer bestimmt werden.
Biochem/physiol Actions
Erkennt Neurofilament-Heavy (NF-H). Der Antikörper ergibt eine scharf definierte Färbung der Filamente im Zytoplasma In Neuronen und insbesondere in Axonen.
Physical form
Format: Aufgereinigt
Purified chicken IgY in PBS containing 5mM sodium azide.
Analysis Note
Kontrolle
Positivkontrolle: Gehirn.
Negativkontrolle: Niere, Leber.
Positivkontrolle: Gehirn.
Negativkontrolle: Niere, Leber.
Other Notes
Konzentration: Die chargenspezifische Konzentration entnehmen Sie bitte dem Analysenzertifikat.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100, 300 |
| biological source chicken | biological source mouse | biological source mouse | biological source mouse |
| antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form ascites fluid | antibody form purified immunoglobulin |
| clone polyclonal | clone NE14, monoclonal | clone N52, monoclonal | clone N52, monoclonal |
| technique(s) immunocytochemistry: suitable, western blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | technique(s) immunoblotting: 1.25-2.5 μg/mL using rat brain S1 fraction, immunohistochemistry: 5-10 μg/mL using enzyme treated formalin-fixed, paraffin-embedded rat brain or mouse brain sections | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): 1:400 using rat cerebellum, microarray: suitable, western blot: 1:1,000 using rat brain extract | technique(s) immunohistochemistry: suitable, western blot: suitable |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in wet ice |
Still not finding the right product?
Explore all of our products under Anti-Neurofilament H Antikörper
— oder —
Probieren Sie unser Produkt-Auswahlhilfe, um Ihre Auswahl einzugrenzen
Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Verwandter Inhalt
Computation identifies structural features that govern neuronal firing properties in slowly adapting touch receptors.
Lesniak, DR; Marshall, KL; Wellnitz, SA; Jenkins, BA; Baba, Y; Rasband, MN; Gerling, GJ; Lumpkin, EA
eLife null
Zu-Lin Chen et al.
Developmental biology, 327(1), 158-168 (2009-01-03)
Laminins have dramatic and varied actions on neurons in vitro. However, their in vivo function in brain development is not clear. Here we show that knockout of laminin gamma1 in the cerebral cortex leads to defects in neuritogenesis and neuronal
Lucas M Viana et al.
Hearing research, 327, 78-88 (2015-05-24)
Recent animal work has suggested that cochlear synapses are more vulnerable than hair cells in both noise-induced and age-related hearing loss. This synaptopathy is invisible in conventional histopathological analysis, because cochlear nerve cell bodies in the spiral ganglion survive for
Global Trade Item Number
| SKU | GTIN |
|---|---|
| AB5539 | 04053252506338 |
-
I am staining FFPE postmortem human spinal cord tissues. What does it mean if some motor neuron cell bodies have intense NF-H signal? Also should I interpret weak signals in the white and grey matter as background? Dendrites? Degenerating axons?
1 answer-
Helpful?
-
-
Does this antibody detect phosphorylated or non-phosphorylated NFH or both?
1 answer-
This product is a polyclonal antibody that was made against biochemically isolated NF-H purified from bovine spinal cord. This preparation is dominated by axonal forms of NF-H which are heavily phosphorylated on the multiply repeated NF-H KSP type sequences, and this antibody reacts very strongly with these phosphorylated repeats. Reactivity with non-phosphorylated KSP sequences is orders of magnitude weaker. In most species there is some cross-reactivity with the phosphorylated KSP sequences found in the related neurofilament subunit NF-M which are similar but not identical to those of NF-H. The antibody recognizes phosphorylated NF-H strongly in all mammals tested to date.
Helpful?
-