AB5374

Anti-Potassium Channel Kir2.1 Antibody

Chemicon^®, from rabbit

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 7

Eigenschaften

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_000891.2
• UniProt accession no.: P63252
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KCNJ2(3759)

Beschreibung

Immunogen

Purified peptide from human Kir2.1 (amino acids 392-410) (Accession number P48049).

Application

Detect Potassium Channel Kir2.1 using this Anti-Potassium Channel Kir2.1 Antibody validated for use in IH & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blot: 1:200 using ECL on rat brain and rat heart membranes. Note: Addition of 0.1% Tween to the standard milk block is recommended for this lot.

Immunohistochemistry on rat heart sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Kir2.1 protein (IRK1, Kcnj2). The epitope is specific for Kir2.1 and is not present in any other known protein.

SPECIES REACTIVITIES: The immunogen sequence is identical in rabbit, bovine, porcine and guinea pig. It is highly conserved in rat and mouse (17/19 residues), chick (15/19 residues), and pigeon (14/18). Other species have not been tested.

Physical form

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Preparation Note

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3