605160

Thrombin, Plasminogen-Free, Bovine

• Synonym(e): Thrombin, Plasminogen-Free, Bovine
• CAS-Nummer: 9002-04-4
• MDL number: MFCD00236878
• NACRES: NA.25
• UNSPSC Code: 12352204
• Specific activity: ≥1000 NIH units/mg protein

Eigenschaften

• description: Merck USA index - 14, 9383
• Quality Segment: 100
• form: lyophilized
• specific activity: ≥1000 NIH units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated
• solubility: water: 1 mg/mL
• foreign activity: Plasminogen, none detected (by rocket IEP)
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

Native, bovine thrombin. Key enzyme that activates factor XIII and converts fibronogen into fibrin. Serine protease formed in the plasma during the coagulation process by activation of prothrombin by a complex of Factor Xa, Factor Va, phospholipid, and Ca²⁺. Thrombin cleaves Arg-Gly bonds in specific sequences only. The best characterized activity of thrombin is the conversion of fibrinogen to fibrin in the clotting process. Inhibited by AEBSF (Cat. No. 101500) Antithrombin (Cat. No. 169756), PMSF (Cat. No. 52332), and TLCK (Cat. No. 616382). Note: 1 KU = 1000 units.

Physical form

Lyophilized from 200 mM NaCl, 50 mM sodium citrate, 0.1% PEG-8000, pH 6.5.

Preparation Note

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.

Analysis Note

Complete activation from homogeneous bovine prothrombin by SDS-PAGE

Other Notes

One unit is determined by comparison with a standard curve prepared using the Bureau of Biologics standard thrombin.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Sicherheitshinweise

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable