492899

Nuclease, Staphylococcus aureus

Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups.

• Synonym(e): Nuclease, Staphylococcus aureus
• CAS-Nummer: 9013-53-0
• EG-Nummer: 3.1.31.1 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54
• MDL number: MFCD00131731
• Specific activity: ≥15,000 units/mg protein
• Biological source: Staphylococcus aureus

Eigenschaften

• biological source: Staphylococcus aureus
• Quality Level: 100
• form: lyophilized solid
• specific activity: ≥15,000 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic)
• color: off-white
• solubility: aqueous buffer: 1 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Beschreibung

General description

Native Stapylococcus aureus nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems. Note: 1 KU = 1000 units.

Other Notes

Krupp, G., and Gross, H.J. 1979. Nucleic Acids Res.6, 3481.

One unit is defined as the amount of enzyme that will release a sufficient amount of acid-soluble oligonucleotides to produce an absorbance increase of 1 at 260 nm in 30 min at 37°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable