401116

Human IgG, Fab Fragment, Plasma

• Synonym(e): IgG Fab fragment
• NACRES: NA.77
• UNSPSC Code: 12352202
• Clone: -
• Species reactivity: -
• Application: —
• Citations: 6

Eigenschaften

• biological source: (Human plasma)
• Quality Segment: 100
• assay: ≥95% (SDS-PAGE)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• dilution: (Native IgG Fab fragment derived from normal human plasma IgG.)
• isotype: IgG
• solubility: sterile distilled water: soluble
• shipped in: ambient
• storage temp.: −20°C
• target post-translational modification: unmodified

Beschreibung

General description

Immunoglobulin G (IgG) is the most crucial glycoprotein antibody.[1] It has the longest serum half-life compared to other immunoglobulins.[1] IgG is present in human serum[2][1] and also identified in the bloodstream.[1] It contains four polypeptide chains, wherein the light and heavy chains are linked by disulfide bonds.[2]

Application

Human IgG, Fab Fragment, Plasma has been used in enzyme-linked immunosorbent assay (ELISA).[3]

Biochem/physiol Actions

Immunoglobulin G (IgG) initiates the classical pathway of the complement system and eliminates virus particles and toxins.[2][1] It controls antibody-dependent cell-mediated cytotoxicity (ADCC)[2] and might be correlated to allergy.[4] Digestion of IgG by papain generates fragment antigen-binding (Fab) whereas pepsin digestion of IgG releases crystallizable (Fc) fragment and a single dimeric F(ab)₂.[1]  Maternal IgG is transferred to the fetus via the placenta and is crucial for the immune defense of the neonate against infections.[5] IgG levels in severe acute respiratory syndrome coronavirus (SARS-CoV)2 infected patients may be useful to understand the severity and prognosis of coronavirus disease 2019 (COVID-19).[6]  

Physical form

In 150 mM NaCl, 20 mM sodium phosphate buffer, 0.05% NaN₃, pH 7.4.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies of HIV and HCV.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: nwg